改良的在体循环法用于胰岛素的鼻腔吸收研究

Modification of in situ perfusion in study of nasal absorption of insulin

本文建立了一种适用于胰岛素鼻腔吸收研究的在体实验方法。首先,考察在体鼻循环实验方法对胰岛素的适用性,结果发现,胰岛素存在一定的管路吸附,传统方法不适用于胰岛素的研究;而在循环液中加入0.001%Labrasol可以有效解决胰岛素的管路吸附问题,该改良方法可用于胰岛素鼻腔吸收研究。采用该方法考察p H和药物浓度对胰岛素鼻腔吸收的影响,结果表明,相比于p H 4.5和p H 7.4,药物在p H 6.0的条件下吸收速度最低。同时,胰岛素的鼻腔吸收机制可能是基于浓度梯度的被动扩散。

The objective of this study is to develop an in vitro screening method for nasal absorption of insulin. First, the adaptability of in situ rat nasal perfusion test for the study of insulin was investigated. It was found that insulin was liable to be absorbed on the silicone tube and the traditional method is not suitable. However, addition of 0.001% Labrasol into the perfusate can effectively solve this problem. A modified method suitable for in situ rat nasal perfusion of insulin was established with the addition of 0.001% Labrasol into the perfusate. Using the modified method, effect of p H and drug concentration on the absorption of insulin in the nasal cavity was further investigated. The results suggest that compared with p H 4.5 and p H 7.4, the drug absorption rate was the lowest at p H 6.0. The intranasal absorption mechanism of insulin may be passive diffusion.