椒样薄荷叶片愈伤组织诱导与抗褐变

Induction and Anti-browning of Leaf Callus From Mentha piperita L.

以椒样薄荷为试材,采用单因素试验法开展了基本培养基的筛选试验,并在基本培养基B5中添加不同植物生长调节剂组合和抗褐变剂,分析其对椒样薄荷愈伤组织形成的影响。结果表明:叶片愈伤组织诱导的基本培养基以B5为宜;植物生长调节剂以KT1.0mg·L^(-1)+2,4-D 0.5mg·L^(-1)为宜;以B5+KT 1.0mg·L^(-1)+2,4-D 0.5mg·L^(-1)为培养基时抗褐变剂以活性炭0.2g·L^(-1)为宜,椒样薄荷愈伤组织诱导率达到100%,褐变率为0.0%,愈伤组织生物量达到42.1g·L^(-1),建立了较理想的愈伤组织体系。

Taking Mentha piperita L.as test material,the effect of callus induction on the leaves of Mentha piperita L.plantlets was studied to provide the basis for establishment of suspension system and regeneration system.The basice mediums,the plant regulator combination with 6-BA,KT and2,4-D,NAA and antibrowning agents were detected for the callus induction and formation.The results showed that B5 as the basic medium and plant growth regulator KT 1.0 mg·L^-1＋2,4-D 0.5 mg·L^-1 combination were suitable for callus induction and formation;the planet leaves callus induction rate was 100%,browning rate was 0.0%,the callus biomass reached 42.1 g·L^-1 on the medium of B5＋KT 1.0 mg·L^-1＋2,4-D 0.5 mg·L^-1 with AC 0.2 g·L^-1.The callus system was established by this experiment.