喉癌病例中HPV感染状态的初步探讨

The infection status of human papillomavirus in patients with laryngeal carcinoma

目的:对喉癌病例中人乳头状瘤病毒(human papillomavirus,HPV)的感染状况进行初步探讨。方法:收集40例新发喉鳞状细胞癌患者(均为广东地区患者)的临床数据及新鲜组织标本,提取DNA,利用Digene HC2 HPV DNA test试剂盒检测组织标本中的HPV DNA。结果:40例喉癌肿瘤及其癌旁组织标本均为HPV阴性。结论:喉癌病例中HPV感染率低。未来仍需进行进一步大规模HPV感染率的检测。

Objective：To investigate the infection status of human papillomavirus （HPV） in patients with laryngeal carcinoma. Methods：Forty primary laryngeal carcinoma patients were enrolled, all of whom came from Guangdong Province. The HPV DNA expression was detected using Digene HC2 HPV DNA test（Qiagen） following the manufae-turer＇s protocol. Results ：None of the DNA sample showed greater HPV DNA level compared with the positive control, suggesting all the included laryngeal carcinoma eases were HPV negative. Conclusion：Clinical HPV infection rate of laryngeal carcinoma was low. Further studies on proving that HPV is not the major risk factor for laryngeal malignan-cies are warrant.