拟南芥ABI3基因缺失突变体的鉴定及对盐胁迫的响应

Identification of ABI3 gene deletion mutant in Arabidopsis thaliana and its response to salt stress

采用PCR和RT-PCR技术分别在DNA和RNA水平上对由于T-DNA插入所导致的拟南芥ABI3基因突变体进行鉴定,鉴定后将野生型Col-0与纯合abi3突变体种子分别置于含不同浓度NaCl的MS培养基上培养,测定萌发率、生物量和根长。结果表明:在NaCl胁迫下,野生型Col-0和纯合abi3突变体种子的萌发率、生物量和根长均随着NaCl浓度的增加呈现下降趋势。在相同NaCl浓度下,纯合abi3突变体种子的萌芽率、生物量和根长均低于野生型Col-0。当NaCl浓度达到150 mmol/L时,纯合abi3突变体种子的萌芽率、生物量和根长均降到最低,与野生型Col-0存在极显著差异。可见,拟南芥ABI3基因具有提高拟南芥抗NaCl胁迫能力的作用。

At the level of DNA and RNA,the Arabidopsis thaliana mutant of ABI3 gene induced by T-DNA insertion was identified by PCR and RT-PCR, respectively. After the identification, the seeds of wild type Col-0 and homozygous mutant abi3 were cultured in MS medium containing different concentrations of NaCl, and the germination rate, biomass and root length were measured. The results showed that under NaCl stress, the germination rate,biomass and root length of wild type Col-0 and homozygous mutant abi3 showed a downward trend with the increase of NaCl concentration. At the same NaCl concentration,the germination rate,biomass and root length of homozygous mutant aba3 were lower than those of wild type. When the concentration of NaCl reached 150 mmol/L,the germination rate,biomass and root length of the homozygous mutant abi3 were all the lowest,and there was a very significant difference between homozygous mutant abi3 and wild type. Therefore,ABI3 gene could enhance the salt tolerance ability of Arabidopsis thaliana.