乳腺癌紫杉醇耐药细胞中lncRNA和mRNA表达谱筛选

Screening of lncRNA and mRNA expression profiles in paclitaxel-induced breast cancer MCF-7 resistant cells

目的:分析紫杉醇诱导的乳腺癌MCF-7耐药细胞(MCF-7/PR)中长链非编码RNA(lnc RNA)和信使RNA(mRNA)表达谱的变化,研究筛选出调控乳腺癌紫杉醇耐药的关键lncRNAs,为逆转紫杉醇耐药的分子靶点提供理论依据。方法:利用Arraystarlnc RNA芯片技术检测MCF-7/PR细胞中lncRNAs和mRNAs的表达谱;使用Agilent Gene Spring GX v12.1软件筛选差异表达的lncRNAs和mRNAs,对差异mRNAs进行GO和KEGG通路分析,得到mRNA参与的生物学途径;并同时进行lncRNAs与相应mRNAs联合分析推断lncRNAs功能。结果:通过MCF-7/PR细胞和MCF-7亲本细胞相比,共有1 504个lncRNAs和2264个mRNAs存在差异性表达(差异倍数>2.0);通过GO聚类分析表明:上调的mRNAs和下调的mRNAs分别参与多种不同的生物过程;差异性lncRNAs可能通过影响相应mRNAs发挥其生物学功能。结论:乳腺癌紫杉醇耐药细胞中lncRNAs和mRNAs的表达存在差异,差异表达的基因可以为寻找新的化疗敏感靶点或生物标志物提供线索。

Objective：To study the changes of long non-coding RNA （IncRNA） and messenger RNA （mRNA） expression profiles in paclitaxel-induced breast cancer MCF-7 resistant cells （MCF-7/PR） ,and to screen the key IncRNAs that regulate the drug resistance of breast cancer cells to paclitaxel, providing theoretical basis for the molecular target of reversing paclitaxel resistance. Methods：The expression profiles of IncRNAs and mRNAs in MCF-7/PR cells were detected by ArraystarlncRNA microarray. Ditterentially expressed lncRNAs and mRNAs were selected using Agilent GeneSpring GX v12.1 software, and GO and KEGG pathways were analyzed for differential mRNAs to find biological pathway involved in mRNAs Simuhaneous analysis of IncRNAs and corresponding mRNAs was per-formed to speculate the function of IncRNAs. Results：A total of 1 504 IncRNAs and 2 264 mRNAs were differentially expressed in MCF-7/PR cells compared with MCF-7 parental cells（ Fold change 〉 2.0）. GO clustering analysis shouted that up-regulated mRNAs and down-regulated mRNAs were involved in maW different biological processes. Further analysis revealed that differential IncRNAs might exert their biological functions by affecting the corresponding mRNAs. Conclusions ：This study finds that the expression of IncR-NAs and mRNAs in paclitaxel-resistant cells of breast cancer is different. Differentially expressed genes can provide clues for finding new sensitive targets or biomarkers for chemotherapy.