滩羊尾脂组织候选基因m RNA表达量分析研究

mRNA Expression of the Candidate Genes Associated with Tail Fat Deposition in Tail Fat Tissue of Tan Sheep

为进一步筛选与滩羊脂肪沉积相关的候选基因,对利用基因芯片扫描和选择信号检测得到的滩羊尾脂组织中与脂肪合成代谢相关的6个重要候选基因(PDGFD、CAV1、PPARG、HSL、PLA2G16、RETN)进行m RNA表达量研究。结果表明,6个基因在滩羊尾脂中均有表达,其中,HSL基因的mRNA表达量显著高于其他基因(P<0.05)。研究结果为滩羊尾型选育及品种改良提供了参考。

In an attempt to further screen the candidate genes associated with tail fat deposition of Tan sheep, the mRNA expression of six candidate genes （PDGFD, CAV1, PPARG, HSL, PLA2G16, RETN） screened by genechip scanning and selection signatures detection was quantitatively assessed. The mRNA expression of the six genes were simultaneously observed in the tail fat tissue of Tan sheep, and the significantly higher mRNA expression was found in HSL gene （P〈0.05）. The results obtained in this study provide valuable references for tail type selection and variety improvement of Tan sheep.