右归丸及其拆方对肾阳虚大鼠血清T、LH和CORT的影响

Effects of Yougui Pill and Its Components on Serum T, LH, and CORT in Rats with Kidney Yang Deficiency

目的探讨右归丸及其拆方对肾阳虚大鼠血清中睾酮(testosterone,T)、促黄体生成素(luteinizing hormone,LH)和皮质醇(cortisol,CORT)的影响。方法取3月龄SPF级雄性SD大鼠50只,随机分成正常对照组、模型组、补阴药组、补阳药组、全方组,除正常对照组外,其他组均采用后肢肌内注射氢化可的松建立肾阳虚动物模型。造模成功后,补阴药组、补阳药组、全方组分别给予相应药物连续灌胃14 d,正常对照组与模型组给予等容量的生理盐水灌胃,停药后第2天,经麻醉后断头处死,心脏取血获得血清,摘除双侧睾丸。记录大鼠体质量及睾丸质量,并计算睾丸指数;用ELISA法测定血清中T、LH和CORT含量。结果模型组大鼠较正常对照组大鼠体质量、睾丸指数及血清T、LH、CORT含量均降低(P<0.05);治疗组中全方组较模型组大鼠大鼠体质量、睾丸指数及血清T、LH、CORT含量明显提升(P<0.05),补阴药组相对补阳药组增加大鼠体质量及血清LH含量(P<0.05),补阳药组相对补阴药提升睾丸指数及血清T含量作用较强(P<0.05);补阴组和补阳组对血清CORT含量均无明显作用(P>0.05)。结论右归丸全方可对肾阳虚证大鼠体质量、睾丸指数、血清中的T、LH和CORT表达水平产生良性影响,右归丸方中的补阴药在提升肾阳虚大鼠体质量和增加血清中LH的含量方面调控作用较好,补阳药在增加血清中T的含量和提高睾丸指数方面调控作用较好,补阴药和补阳药对血清中CORT表达水平的调控均无明显作用。

Objective To explore the effect of Yougui Pill and its components on sermn testosterone （T）, luteinizing homlone （Lid）, and eortisol （CORT） in rats with kidney Yang deficiency-. Methods A total of 50 3-month-old specific pathogen-free nmle Sprague-Dawley rats were randomly divided into nomal control group, model group, Yin nourishing component group, Yang tonify-ing component group, and whole prescription group. All rats except those in the nomal control group were intramuscularly injected with hydrocortisone in the hind legs to establish an animal model of kidney Yang deficiency. After completion of model establishment, the rats were treated for 14 consecutive day-s as follows： intragastric administration of con＇esponding medicines fbr the Yin nourishing component group, the Yang tonify-ing component group, and the whole prescription group; intragastricadnfinistration of an equal amount of nomal saline for the normal control group and the model group. On the second day of medicine discontinuation, the rats were anesthetized and decapitated; serum was collected by cardiac puncture, and both testes were also collected. Body weight and testis weight of the rats were recorded, and testieular index was calculate＆ ELISA was used to detemfine the levels of T, LH, and COtlT. Results Compared with the nomlal control group, the model group had significantly reduced body weight, testieular index, and serum levels of T, LH, and COtlT （P〈0.05）. The whole prescription group had significantly increased body- weight, testieular index, and sermn levels of T, LH, and CORT compared with the model group （P〈0.05）. Compared with the Yang tonity-ing component group, the Yin nourishing component group had significantly greater increases in body weight and sermn LH level （P〈0.05）, but significantly less increases in testieular index and sermn T level （P〈0.05）. Neither of the above two groups showed a significant change in sermn COtlT level （P〉0.05）. Conclusion The whole prescription of Yougui Pill has favorable etteets on body- weight, testieular index, and serum levels of T, LH, and COtlT in rats with kidney Yang deficiency; Yin nourishing component in the prescription reveals good etteets in increasing body weight and serum LH level, and Yang tonity-ing component reveals good etteets in increasing testieular index and serum T level; neither of the above components has an obvious etteet in regulating the level of sermn CORT.