摘要
目的:检测经不同浓度的恩度处理后Her-2过表达乳腺癌细胞MDA-MB-453中pAKT的表达量,初步探讨pAKT在恩度诱导的肿瘤细胞凋亡中的作用。方法:常规体外培养MDA-MB-453细胞,分别用浓度为0μg/ml、200μg/ml、400μg/ml、800μg/ml的恩度处理细胞24 h,Western Blot法检测各浓度恩度处理后细胞的pAKT表达量。结果:随恩度浓度增加,细胞中pAKT表达量逐渐下降。与0μg/ml恩度组相比,200μg/ml组与之无统计学差异(P=0.306),400μg/ml和800μg/ml组与之比较均有统计学差异(P<0.05),但400μg/ml组与800μg/ml组之间无统计学差异(P=0.91)。结论:pAKT表达下调可能参与介导恩度诱导的Her-2过表达乳腺癌细胞凋亡。
Objective :To elicit the role of pAKT placed in the apoptosis of Her-2 over-expressed breast cancer cells induced by Endostar. Methods:Cultivate MDA - MB -453 cells in vitro,and treat each group with Endostar for 24 h,then quantify pAKT expressed in cells of each group by Western Blot method. Results:The expression of pAKT in cells descended with the ascending concentration of Endostar. There was no significant difference between 0μg/ml group and 200 ixg/ml group (P = 0. 306 ). But pAKT was statistically reduced in 400 μg/ml group and 800 μg/ml group ( P 〈 0.05 ) ,while the difference between these two groups was tiny ( P = 0.91 ). Conclusion : Down - regulated of pAKT may be one of those mechanisms uderlying the apoptosis of Her - 2 over - expressed breast cancer cells induced by Endostar.
作者
张世军
李里
金英华
申维喜
姜秋颖
韩楠楠
于静
高雨歌
Zhang Shijun;Li Li;Jin Yinghua;Shen Weixi;Jiang Qiuying;Han Nannan;Yu Jing;Gao Yuge(Department of Oncology,The General Hospital of Dongfeng Aj:filiated to Hubei Medical University,Hubei Shiyan 442001,China;Department of Medical Oneology,The Second Affiliated Hospital of Harbin Medical University,Heilongjiang Harbin 150086,China.)
出处
《现代肿瘤医学》
CAS
2018年第19期3030-3032,共3页
Journal of Modern Oncology
基金
哈尔滨市应用技术研究与开发项目(编号:2013RFXYJ069)