全氟己烷脂质体纳米粒在超声造影方面价值的实验研究

The application assessment of perfluorohexane based liposome nanoparticles on ultrasound enhanced imaging

目的:研究并评价基于全氟己烷的新型纳米超声造影剂的可行性。方法:本实验首先设计并成功制备了一种全新的全氟己烷(PFH)-光敏剂(IR780)脂质体纳米粒[LIP(PFH+IR780)]。该纳米粒可在近红外光激发下,由IR780产热升温,触发PFH气化产生大量微泡。对LIP(PFH+IR780)进行粒径测定、光镜成像,明确性质。并在体外及体内对该纳米粒子进行超声成像,验证LIP(PFH+IR780)的超声增强效果。结果:通过体外表征,证实该纳米粒粒径为250 nm左右。经过体外超声实验发现,LIP(PFH+IR780)较对照组使得超声回声明显增强。荷瘤小鼠体内实验发现,LIP(PFH+IR780)可以有效蓄积在肿瘤部位,并且同样使得超声图像得到显著增强。结论:LIP(PFH+IR780)纳米粒子在超声造影成像上具有一定的应用价值。

Objective：To explore whether perflexane based liposome nanoparlicles can be used as ultrasound contrast agent. Methods：Firstly,we had designed and synthesized a creatively size -variable liposome nanoparficle I LIP （PFH ＋ IR780） ] with perfluorohexane（PFH） liquid and photosensitizer IR780 loaded in. When irradiated by near - ilffrared light last for 1 rain, PFH nano-droplet vaporized into micro - bubble by heated from IR780. Then we conducted experimental study on ultrasound images both in vitro and in vivo, and analyzed data finally. Results：After successful synthesis and size measurement, the diameter of LIP（ PFH ＋ IR780） reached to about 250 nm that was easier to accumulated into tumor under enhanced permeabiliU and retention（EPR） effect. Through ultrasound experiment in vitro,we found a more enhanced ultrasound image was made by LIP（ PFH ＋ IR780） than others,as same as the experiment in vivo. Conclusion：Hence, perfluorohexane based liposome nanoparticles have a promising application prospective on ultrasound enhanced imaging.