放射性涎腺损伤中自噬基因Beclin 1的表达及其临床意义

Expression and significance of Beclin 1 in radiation-induced salivary gland injury

目的:探讨自噬基因Beclin 1在放射性涎腺损伤组织中的表达及其临床意义。方法:收集2010年6月至2016年9月海南省人民医院颌面外科临床资料和病理科存档的115例下颌下腺组织蜡块,其中正常下颌下腺25例(正常组),下颌下腺炎组25例,下颌下腺退行性改变组25例,放射性下颌下腺炎组21例,放射性下颌下腺退行性改变组19例,采用免疫组化法检测各组Beclin 1蛋白的表达。结果:在涎腺炎性病变组织中,放射性下颌下腺炎组Beclin 1蛋白的阳性表达率明显高于正常组和下颌下腺炎组(均P<0.05);在涎腺退行性病变组织中,放射性下颌下腺退行性改变组Beclin 1蛋白的阳性表达率明显高于正常组和下颌下腺退行性改变组(均P<0.05)。结论:放射性损伤能明显增加涎腺组织中Beclin 1蛋白的表达,自噬可能参与了涎腺放射性损伤的进展。

Objective： To investigate the expression and significance of autophagy-related gene Beclin 1 in radiation-induced salivary gland injury tissue.Methods： A total of 115 paraffin-embedded blocks including 25 samples with submandibular adenitis,25 submandibular gland degeneration,21 radioactive submandibular adenitis,19 radioactive submandibular gland degeneration and 25 specimens of normal salivary gland as control were studied.Immunohistochemical method was used to determine the expression of Beclin 1 in submandibular gland tissues.Results： The positive expression rate of Beclin 1 was significantly higher in radioactive submandibular gland group than that in the control group and submandibular adenitis group （ P 〈0.05）.Compared with the control group and the submandibular gland degeneration group,the Beclin 1 expression was up-regulated in the radioactive submandibular gland degeneration group （ P 〈0.05）.Conclusion： Radioactive injury could markedly increase the expression of Beclin 1 in salivary gland tissue,suggesting that autophagy may be involved in the progress of radiation-induced salivary gland injury.