摘要
N^6-methyladenosine （m^6A） is the most common post-transcriptional RNA modification throughout the transcriptome, affecting fundamental aspects of RNA metabolism, m^6A modification could be installed by m^6A ＂writers＂ composed of core catalytic components （METTL3/METTL14/WTAP） and newly defined regulators and removed by m^6A ＂erasers＂ （FTO and ALKBH5）. The function of m^6A is executed by m^6A ＂readers＂ that bind to m^6A directly （YTH domain-containing proteins, eIF3 and IGF2BPs） or indirectly （HNRNPA2B1）. In the past few years, advances in m^6A modulators （＂writers,＂ ＂erasers,＂ and ＂readers＂） have remarkably renewed our understanding of the function and regulation of m^6A in different cells under normal or disease conditions. However, the mechanism and the regulatory network of m^6A are still largely unknown. Moreover, investigations of the m^6A physiological roles in human diseases are limited. In this review, we summarize the recent advances in m^6A research and highlight the functional relevance and importance of m^6A modification in in vitro cell lines, in physiological contexts, and in cancers.
N^6-methyladenosine （m^6A） is the most common post-transcriptional RNA modification throughout the transcriptome, affecting fundamental aspects of RNA metabolism, m^6A modification could be installed by m^6A ＂writers＂ composed of core catalytic components （METTL3/METTL14/WTAP） and newly defined regulators and removed by m^6A ＂erasers＂ （FTO and ALKBH5）. The function of m^6A is executed by m^6A ＂readers＂ that bind to m^6A directly （YTH domain-containing proteins, eIF3 and IGF2BPs） or indirectly （HNRNPA2B1）. In the past few years, advances in m^6A modulators （＂writers,＂ ＂erasers,＂ and ＂readers＂） have remarkably renewed our understanding of the function and regulation of m^6A in different cells under normal or disease conditions. However, the mechanism and the regulatory network of m^6A are still largely unknown. Moreover, investigations of the m^6A physiological roles in human diseases are limited. In this review, we summarize the recent advances in m^6A research and highlight the functional relevance and importance of m^6A modification in in vitro cell lines, in physiological contexts, and in cancers.
基金
This work was supported by the National Natural Science Foundation of China （No. 91753141 to Hua-Bing Li）.
