摘要
N^6-methyladenosine (m^6A) is the most common post-transcriptional RNA modification throughout the transcriptome, affecting fundamental aspects of RNA metabolism, m^6A modification could be installed by m^6A "writers" composed of core catalytic components (METTL3/METTL14/WTAP) and newly defined regulators and removed by m^6A "erasers" (FTO and ALKBH5). The function of m^6A is executed by m^6A "readers" that bind to m^6A directly (YTH domain-containing proteins, eIF3 and IGF2BPs) or indirectly (HNRNPA2B1). In the past few years, advances in m^6A modulators ("writers," "erasers," and "readers") have remarkably renewed our understanding of the function and regulation of m^6A in different cells under normal or disease conditions. However, the mechanism and the regulatory network of m^6A are still largely unknown. Moreover, investigations of the m^6A physiological roles in human diseases are limited. In this review, we summarize the recent advances in m^6A research and highlight the functional relevance and importance of m^6A modification in in vitro cell lines, in physiological contexts, and in cancers.
N^6-methyladenosine (m^6A) is the most common post-transcriptional RNA modification throughout the transcriptome, affecting fundamental aspects of RNA metabolism, m^6A modification could be installed by m^6A "writers" composed of core catalytic components (METTL3/METTL14/WTAP) and newly defined regulators and removed by m^6A "erasers" (FTO and ALKBH5). The function of m^6A is executed by m^6A "readers" that bind to m^6A directly (YTH domain-containing proteins, eIF3 and IGF2BPs) or indirectly (HNRNPA2B1). In the past few years, advances in m^6A modulators ("writers," "erasers," and "readers") have remarkably renewed our understanding of the function and regulation of m^6A in different cells under normal or disease conditions. However, the mechanism and the regulatory network of m^6A are still largely unknown. Moreover, investigations of the m^6A physiological roles in human diseases are limited. In this review, we summarize the recent advances in m^6A research and highlight the functional relevance and importance of m^6A modification in in vitro cell lines, in physiological contexts, and in cancers.
基金
This work was supported by the National Natural Science Foundation of China (No. 91753141 to Hua-Bing Li).