摘要
Methane production from low-strength wastewater （LSWW） is generally difficult because of the low metabolism rate of methanogens. Here, an up-flow biofilm reactor equipped with conductive granular graphite （GG） as fillers was developed to enhance direct interspecies electron transfer （DIET） between syntrophie electroactive bacteria and methanogens to stimulate methanogcnesis process. Compared to quartz sand fillers, using condnctive fillers significantly enhanced methane production and accelerated the start-up stage ofbJ.ofilm reactor. At HRT of 6 h, the average methane production rate and methane yield of reactor with GG were 0.106 m3/（m3.d） and 74.5 L/kg COD, which increased by 34.3 times and 22.4 times respectively compared with the reactor with common quartz sand fillers. The microbial cornmunity analysis revealed that methanogens structure was significantly altered and the archaea that are involved in DIET （such as Methanohacterium） were enriched in GG fillet- The beneficial effects ot conductive fillers on methane production implied a practical strategy tbr efficient methane recovery from LSWW.
Methane production from low-strength wastewater （LSWW） is generally difficult because of the low metabolism rate of methanogens. Here, an up-flow biofilm reactor equipped with conductive granular graphite （GG） as fillers was developed to enhance direct interspecies electron transfer （DIET） between syntrophie electroactive bacteria and methanogens to stimulate methanogcnesis process. Compared to quartz sand fillers, using condnctive fillers significantly enhanced methane production and accelerated the start-up stage ofbJ.ofilm reactor. At HRT of 6 h, the average methane production rate and methane yield of reactor with GG were 0.106 m3/（m3.d） and 74.5 L/kg COD, which increased by 34.3 times and 22.4 times respectively compared with the reactor with common quartz sand fillers. The microbial cornmunity analysis revealed that methanogens structure was significantly altered and the archaea that are involved in DIET （such as Methanohacterium） were enriched in GG fillet- The beneficial effects ot conductive fillers on methane production implied a practical strategy tbr efficient methane recovery from LSWW.
基金
This research was supported by the National Natural Science Foundation of China （Grant No. 51578534）, and Youth Innovation Promotion Association CAS （No. 2017062）.
