奥曲肽对胃癌细胞增殖和凋亡的作用

The effect of octreotide on the proliferation and apoptosis of gastric cancer cells

[目的]观察奥曲肽对胃癌细胞增殖、凋亡、细胞周期的影响,探讨奥曲肽抑癌的可能作用机制。[方法]应用MTT方法检测不同浓度奥曲肽在不同时间对胃癌BGC823细胞的生长抑制作用;采用流式细胞技术检测奥曲肽对胃癌BGC823细胞周期及凋亡情况的影响;利用RT-PCR、Real Time PCR和Western blot等方法明确奥曲肽作用于胃癌BGC823细胞后胃泌素基因及蛋白水平的表达变化。[结果]MTT细胞实验显示,奥曲肽对胃癌BGC823细胞的生长和增殖有抑制作用,且存在量效、时效关系和饱和性;流式细胞技术检测结果显示奥曲肽有明显的促进细胞凋亡的作用,使细胞周期阻滞于G1/S期;RT-PCR和Real-time PCR、Western blot结果显示奥曲肽作用于胃癌BGC823细胞后胃泌素的表达变化不明显,蛋白水平表达与基因水平一致。[结论]奥曲肽抑制胃癌增殖可能是通过细胞周期阻滞、诱导细胞凋亡实现的,奥曲肽没有抑制胃泌素表达的作用。

[Objective]To observe the effect of octreotide on the proliferation,apoptosis and cell cycle of gastric cancer cells,and to explore the possible mechanism of octreotide to inhibit cancer.[Methods]The effect of different concentrations of octreotide in different times on gastric cancer BGC823 cells were detect by MTT assay.Effect of octreotide on the cell cycle and apoptosis of BGC823 gstric cancer cell line by flow cytometry.RT-PCR,Real Time PCR and Western blot were used to determine the growth inhibitory effect of octreotide on gastric cancer BGC82.[Results]MTT assay showed that octreotide inhibited the growth and proliferation of gastric cancer BGC823 cells in a dose-dependent,time-dependent relationship and saturation.Flow cytometry showed that octreotide has obvious role in promoting cell apoptosis,cell cycle arrest in G1/S phase.RT-PCR and Real-time PCR and Western blot showed that octreotide is not obvious changes in the expression of gastrin in gastric cancer BGC823 cells,the protein expression is consistent with the gene level.[Conclusion]Octreotide inhibits the proliferation of gastric cancer by cell cycle arrest and induced apoptosis,and octreotide does not inhibit the expression of gastrin.