miR-622在结肠癌组织中的表达水平与患者临床病理特征及其预后的关系分析

Expression of miR-622 in colon cancer tissues and its relationship with clinicopathological features and prognosis

目的探讨miR-622在结肠癌组织中的表达水平与患者临床病理特征及预后的关系。方法收集2010年1月至2011年6月手术切除的110例结肠癌组织及癌旁正常组织标本,采用实时荧光定量PCR(qRT-PCR)检测组织标本中miR-622的表达情况,分析miR-622与患者临床病理特征的关系,并应用Kaplan-Meier法分析miR-622与结肠癌患者预后的关系。结果结肠癌组织中miR-622的表达水平低于癌旁正常组织(P<0.05),结肠癌组织中miR-622的表达水平与患者血管淋巴管浸润、淋巴结转移、远处转移及临床分期有关(均P<0.05),与性别、年龄、肿瘤大小、分化程度及浸润深度无关(均P> 0.05)。KaplanMeier生存分析显示miR-622低表达的结肠癌患者预后较高表达者更差﹙P<0.05﹚。结论 miR-622的表达水平与结肠癌的发生、发展及预后有关,miR-622或有望成为结肠癌患者预后评估的潜在标志物。

Objective To investigate the expression of miR-622 in colon cancer tissues and its relationship with clinicopathological features and prognosis. Methods 110 resected colon cancer tissue and para-carcinoma normal tissue specimens were collected from January 2010 to June 2011. The expression of miR-622 in tissues was detected by q RT-PCR. The correlation between miR-622 expression and clinicopathological features was analyzed. Kaplan-Meier analysis was used to investigate the relationship between miR-622 and prognosis. Results miR-622 expression level in colon cancer tissue was significantly lower than in para-carcinoma normal tissue（P〈0.05）. miR-622 expression in colon cancer tissue were significantly related to vascular invasion, lymph node metastasis,distant metastasis and clinical stage（P〈0.05）, but it was not related to sex, age, tumor size, differentiation degree and depth of invasion（P〈0.05）. Kaplan-Meier survival analysis showed that the prognosis of patients with low expression of miR-622 was worse than those with high expression（P〈0.05）. Conclusion Decreased expression of miR-622 was associated with the occurrence, development and prognosis of colon cancer. miR-622 may be a potential indicator for prognosis in patients with colon cancer.