miR-502对宫颈癌Hela细胞增殖和转移的影响

Effect of miR-502 on proliferation and metastasis in cervical cancer cell Hela

目的探讨miR-502对宫颈癌患者预后及其对宫颈癌Hela细胞增殖和转移的影响。方法通过数据库分析miR-502表达与宫颈癌患者临床病理特征的关系及其对预后的影响。在Hela细胞中过表达miR-502-3p后,采用CCK-8检测细胞增殖情况,Transwell实验检测细胞迁移和侵袭情况。结果数据库分析结果显示,高表达miR-502的宫颈癌患者总生存期更长,但与TNM分期无明显相关性。CCK-8检测结果显示,miR-502-3p类似物组细胞增殖率低于miR-502-3p抑制剂组[(78.82±1.41)% vs (124.06±7.72)%,P<0.05];Transwell实验检测结果显示,miR-502-3p类似物组细胞迁移率较miR-502-3p抑制剂组低[(85.39±7.19)% vs (121.17±8.20)%,P<0.05],细胞侵袭率亦较低[(85.32±7.12)% vs (117.13±7.37)%,P<0.05]。结论高表达miR-502的宫颈癌患者预后较好,可能与其抑制宫颈癌细胞增殖和转移有关。

Objective To investigate the effect of miR-502 on prognosis of patients with cervical cancer,and its effect on the proliferation and metastasis in cervical cancer cell Hela. Methods The relationship between miR-502 and clinicopathological characteristics of cervical cancer patients was analyzed. After overexpression of miR-502 in Hela cells,the proliferation of cells was measured by cell counting kit-8（CCK-8）,while cell migration and invasion was detected using Transwell assay. Results Patients with high expression of miR-502 showed a better overall survival,but it did not correlated with tumor stage. Compared with the miR-502-3p suppressing group,the miR-502-3p analogues group significantly inhibited cell proliferation[（78.82±1.41）% vs （124.06±7.72）%,P〈0.05],cell mi gration[（85.39 ±7.19）% vs （121.17 ±8.20）%,P〈0.05],and cell invasion [（85.32 ±7.12）% vs （117.13 ±7.37）%,P〈0.05].Conclusions High expression of miR-502 can significantly inhibit proliferation and metastasis by cervical cancer cells,and it may relate to better prognosis of cervical cancer patients.