摘要
目的观察中药复方制剂糖络宁对糖尿病大鼠背根神经节(DRG)线粒体呼吸链氧化呼吸功能、超氧阴离子产生量及膜电位水平的影响。方法选取SPF级雄性8周SD大鼠80只,随机选择14只为正常组,其余66只大鼠采用链脲佐菌素制作糖尿病大鼠模型;其中61只造模成功大鼠采用随机数字表法分为模型组19只、西药组(α-硫辛酸组)21只、中药组(糖络宁组)21只,共给药8周;给药结束后,检测线粒体呼吸链氧化呼吸功能、超氧阴离子产生量及膜电位水平。结果与正常组比较,模型组大鼠DRG线粒体呼吸功能明显下降(P<0.01);与模型组比较,西药组大鼠DRG线粒体呼吸功能明显改善(P<0.01);与模型组比较,中药组大鼠DRG线粒体呼吸功能未见明显改善(P>0.05)。与正常组比较,模型组大鼠DRG线粒体超氧阴离子明显升高(P<0.01);与模型组比较,中药组、西药组大鼠DRG线粒体超氧阴离子水平明显降低(P<0.01)。与正常组比较,模型组大鼠线粒体膜电位明显下降(P<0.01);与模型组比较,中药组、西药组大鼠线粒体膜电位明显改善(P<0.01)。结论糖络宁不能改善糖尿病大鼠DRG线粒体氧化呼吸功能,可抑制糖尿病大鼠DRG线粒体超氧阴离子的产生,改善线粒体膜电位水平,这可能是糖络宁治疗糖尿病周围神经病变的作用机制。
Objective To observe the effect of Chinese herbal compound Tang-luo-ning on oxidative respiratory func- tion of the mitoehondrial respiratory chain, superoxide anion production and membrane potential in diabetic rat dorsal root ganglion (DRG). Methods There were 80 SD male rats, 14 of them were randomly selected as the normal group. The rest 66 were used to established diabetic rats model by injected Streptozoein (STZ). According to the random number table, the 61 rats of the model were divided into model group with 19 rats, Western medicine group (alpha lipoie acid group) with 21 rats and traditional Chinese medicine group (Tang-luo-ning group) with 21 rats. They were given the medicine 8 weeks, then mitoehondrial respiratory chain oxidative respiratory function, superoxide anion production and membrane potential levels were measured. Results Compared with the normal group, the mitoehondrial respiratory function of the DRG in the model group was significantly decreased (P 〈 0.01). Compared with the model group, the mitoehondrial respiratory function of the DRG in the Western medicine group was significantly improved (P 〈 0.01). Compared with the model group, the Chinese medicine group had no significant improvement in mitoehondrial respirato- ry function in DRG(P 〉 0.05). Compared with the normal group, the mitoehondrial superoxide anion of the DRG in the model group was significantly increased (P 〈 0.01). Compared with the model group, the mitoehondrial superoxide anion level of the DRG in the Chinese medicine group and the Western medicine group was significantly reduced (P 〈 0.1). Compared with the normal group, the mitoehondrial membrane potential of the model group was significantly decreased(P 〈 0.01). Compared with the model group, the mito- ehondrial membrane potential of the Chinese medicine group and the Western medicine group was significant- ly improved (P 〈 0.01). Conclusion Tang-luo-ning inhibits the generation of superoxide anion and ameliorates mitochondrial membrane potential in dorsal root ganglions of diabetic rats. This study provides a potential mechanism underlying protection of Tang-luo-ning on the injury of dorsal root ganglions in diabetic model.
作者
刘琴
李逸潇
张涛静
龚燕冰
周晖
谢培凤
LIU QiN;LI Yixiao;ZHANG Taojing;GONG Yanbing;ZHOU Hui;XlE Peifeng(The Second Clinical Medical College of Beijing University of Chinese Medicine,Beijing 100029,China;Depart-ment of Endocrinology,Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078,China)
出处
《中国医药导报》
CAS
2018年第26期4-8,共5页
China Medical Herald
基金
国家自然科学基金面上项目(81473663)
国家自然科学基金青年基金项目(81102574)
关键词
糖络宁
糖尿病大鼠
氧化呼吸功能
超氧阴离子
线粒体
膜电位
Tang-luo-ning
Diabetic rats
Oxidative respiratory function
Superoxide anion
Mitoehondria
Membranepotential