小干扰RNA靶向MIF对胃癌细胞SGC-7901增殖和凋亡的影响

Effect of MIF-targeting small interference RNA on proliferation and apoptosis of gastric cancer cell SGC-7901

目的探讨靶向巨噬细胞移动抑制因子(MIF)的小干扰RNA(siRNA)对胃癌细胞SGC-7901增殖和凋亡的影响。方法取对数生长期的SGC-7901细胞,采用脂质体法分别转染靶向人MIF的siRNA(si MIF组)或阴性对照序列(NC组),48 h后采用Western blotting检测MIF蛋白的表达情况,MTT法观察转染后24、48、72 h的细胞增殖情况,流式细胞仪检测转染后72 h的细胞凋亡率,Western blotting检测凋亡相关蛋白Bcl-2和Bax的表达变化。结果 si MIF组转染48 h后的MIF相对表达量为0.321±0.104,低于NC组的1.078±0.212,差异有统计学意义(P<0.05)。si MIF组转染48~72 h后的细胞增殖率低于NC组,差异有统计学意义(P<0.05)。转染MIF siRNA 72 h后,si MIF组的细胞凋亡率为(23.5±3.6)%,高于NC组的(4.7±1.7)%,差异有统计学意义(P<0.05)。si MIF组Bcl-2的相对表达量为0.663±0.209,低于NC组的1.129±0.178,而Bax相对表达量为0.981±0.225,高于NC组的0.587±0.254,以上差异均有统计学意义(P<0.05)。结论 siRNA靶向沉默MIF能够降低SGC-7901细胞中MIF蛋白表达,抑制SGC-7901细胞的增殖并促进凋亡,在胃癌的靶向治疗中有一定前景。

Objective To investigate the effect of macrophage migration inhibitory factor （MIF）.targeting small interferenceRNA （siRNA） on proliferation and apoptosis of gastric cancer SGC-7901 cells. Methods SGC-7901 cells at logarithmic growth phasewere transfected with human MIF siRNA （siMIF group） or negative control sequence （NC group） by liposome method. Western blot.ting was used to detect the expression of MIF protein at 48 h after transfection. MTT method was used to observe the proliferation at 24,48 and 72 h after transfection. The apoptotic rate was detected by flow cytometry at 72 h after transfection. Western blotting was used todetect the expression of apoptosis.related proteins Bcl-2 and Bax. Results After transfection for 48 h, the relative expression of MIFprotein in siMIF group was 0. 321±0. 104, lower than 1. 078±0. 212 in NC group, and the difference was statistically significant （P〈0. 05）. The proliferative rates of siMIF group were lower than those of NC group at 48.72 h after transfection, and the difference wasstatistically significant （P〈0.05）. After transfection of MIF siRNA for 72 h, the apoptotic rate of siMIF group was （23. 5±3. 6）%,higher than （4. 7±1. 7）% of NC group, and the difference was statistically significant （P〈0. 05）. The relative expression of Bcl.2 insiMIF group was 0. 663±0. 209, lower than 1. 129±0. 178 in NC group, and the relative expression of Bax in siMIF group was 0. 981±0. 225, higher than 0. 587±0. 254 in NC group, and the above difference was statistically significant （P〈0. 05）. Conclusion SiRNAtargeting MIF can reduce the expression of MIF protein in SGC.7901 cells, inhibit the proliferation and promote apoptosis of SGC.7901cells, and it has a certain prospect in the target therapy of gastric cancer.