陆地棉中赤霉素合成途径关键酶基因的时空表达变化

The Temporal Spatial Expression of Gibberell in Key Metabolic Enzyme in Upland Cotton

为揭示赤霉素合成途径关键酶基因在棉花生长发育过程中的调控作用,利用实时荧光定量PCR方法中的相对定量方法,对中棉所49材料植株体内的赤霉素合成途径相关基因表达量进行了检测分析。结果显示,幼苗期茎组织内除GA2ox1基因外,其余各基因表达量相对较高。开花期根中GA2ox1、GA3ox1、GID1B,茎中CPS1、GA20ox1、GA3ox1、GID1B和叶中GA3ox1基因表达量上调;吐絮期根中GA2ox1和GA3ox1基因表达量上调,其余基因下调,茎中KS、GID1B基因表达量下调,其余基因上调,其中以GA2ox1上调幅度最大,叶中GA2ox1基因表达量下调,其余基因上调。结果表明,开花期,茎中GA20ox1基因的高表达为植株茎的伸长及果枝发育提供必要的活性赤霉素水平,GA3ox1基因可能与开花成铃有关;吐絮期,随着根茎组织的木质化,根茎中GA2ox1基因表达量逐渐升高以降低活性GAs合成,而叶组织中GA3ox1和GA20ox1基因表达量上调,则为棉桃生长发育与脱水成熟提供必要的激素水平。棉花通过改变植株体内赤霉素合成代谢途径关键酶基因的表达量来调控植株的生长发育,且随着生长发育的进行,各目的基因的表达量变化趋势存在一定差异。为深入研究赤霉素对陆地棉的调控机理提供了理论基础,有助于加快利用赤霉素进行品种改良与种质创新。

To reveal the relationship between the key enzyme gene expression of gibberellin synthesis pathway and different tissues or stages of growth,the CCRI49 cotton was selected as material, and relative quantification Real-time PCR was developed to determine the gibberellin key gene expression of different cotton organizations in different stages of development. The results showed that, in seedling, the expression level of all gibberellin synthesis pathway genes was higher in stem,except GA2oxl gene. In full-blooming stage,the expression of GA2ox1, GA3ox1, GIDIB in root,CPS1 ,GA2Oox1 ,GA3oxl ,GID1B in stem and GA3ox1 in leaf had an up-regulation. In boll opening stage, the expression levels of GA2ox1 and GA3ox1 were up-regulated in root, while the remaining genes were down-regulated. The expression levels of KS and GIDIB in stem were down-regulated, while the other genes were up-regulated, with GA2ox1 rise in maximum. And GA2ox1 in the leaf was down-regulated and the rest were up-regulated. In full-blooming stage, the high expression of GA2Oox1 gene provided the necessary active gibberellin level for the elongation and growth of the plant stem,and the GA3oxl gene might be associated with flowering and bell. In boll opening stage,the expression of GA2ox1 gene in root and stem gradually increased to reduce the synthesis of active,gibberellic acid. The gene expression levels of GA3ox1 and GA2Oox1 in leaf tissues were raised to provide the necessary hormone levels for cotton boll growth and dehydration. The results indicated that cotton plant by changing the key enzymes gene expression of gibberellin synthesis metabolic pathway to regulate plant growth and development,and the purpose gene expression volume trends exist a certain difference along with the growth and development. It provides a theoretical basis for studying the regulation mechanism of gibberellin on upland cotton,which can help accelerate the improvement of variety and germplasm innovation.