基于SSR标记的杂交大豆主要亲本遗传多样性分析

Genetic Diversity Analysis of Main Parents for Hybrid Soybean Based on SSR Markers

为明确山西已有杂交大豆主要亲本的遗传多样性和亲缘关系,利用64对SSR引物对32份杂交大豆亲本包括15份保持系与17份恢复系进行了遗传多样性分析。结果共检测到357个等位基因变异,单个位点检测到的等位基因数变化为2~12个,平均为5. 578个,其中,保持系检测到322个等位基因变异,变化为2~9个,平均为5. 031个,恢复系检测到320个等位基因变异,变化为2~12个,平均为5个;总的位点多态性信息含量(PIC)变辐为0. 368~0. 900,平均为0. 666,其中,保持系PIC变辐为0. 370~0. 868,平均为0. 661,恢复系PIC变辐为0. 329~0. 904,平均为0. 630。聚类分析结果表明,保持系间的遗传相似系数(GS)平均为0. 637,在相似系数为0. 61处,可将供试的保持系聚为两大类;其中,第Ⅱ类在相似系数为0. 65处又可分为3个亚类;恢复系间的遗传相似系数平均为0. 669,在相似系数为0. 65处,将供试的恢复系聚为两大类;聚类结果与品种地理来源具有一定的相关性。

To determine the genetic diversity and genetic relationship of the main parents of hybrid soybean in Shanxi Province, 64 pairs of SSR primers were used to analyze the genetic diversity of 32 hybrid soybean parents including 15 maintainer lines and 17 restorer lines. Totally 357 alleles were revealed among 32 parents, 2-12 alleles could be detected at different SSR locus, with the average of 5.578 alleles per SSR marker, of which 322 alleles were detected in the maintainer line, ranging from 2 to 9 with an average of 5.031, and the detection of 320 alleles in the restorer ranged from 2 to 12 with an average of 5. The polymorphism information content （PIC）of the total polymorphism was 0.368-0.900, with an average of 0.666, of which, the PIC variation of maintainer lines ranged from 0.370 to 0.868 with an average of 0.661, and the PIC variation of restorer lines ranged from 0.329 to 0.904 with an average of 0.630. Cluster analysis result showed that the average genetic similarity coefficient （GS）between maintainer lines was 0.637. At the genetic similarity coefficient （GS）of 0.61, the maintainer lines could be grouped into two major groups. At the similarity coefficient of 0.65, the second group could be divided into three subcategories. The average genetic similarity coefficient （GS）between restorer lines was 0.669. At the genetic similarity coefficient （GS）of 0.65, the restorer lines could be grouped into two major groups. The clustering results were related to the geographical origin of cultivars.