初花后短日照诱导对小豆叶片生理参数的影响

Effects of Short-day Photoperiod Inducement after Early Flowering on Physiological Parameters in Adzuki Bean Leaves

为了明确初花后短日照诱导对小豆叶片生理参数的影响,以中熟品种冀红9218和晚熟品种唐山红小豆为试验材料,分别在初花-初荚(EF-EP)、初荚-初粒(EP-ES)和初花-初粒(EF-ES) 3个生育时期进行短日照诱导,研究其对小豆叶片生理参数的影响。结果表明,初花后短日照诱导降低2个小豆品种的叶绿素含量,同时,净光合速率也呈现降低趋势,从而导致最大光化学量子产量(Fv/Fm)降低;可溶性糖和可溶性蛋白含量在12 h的EF-ES处理下受短日照诱导的影响较大,导致碳氮代谢速度减慢,此时,蔗糖合成酶(SS)活性呈现降低趋势,可见,碳代谢的速度与蔗糖合成酶(SS)活性有关,但2个品种的蔗糖磷酸合成酶(SPS)活性在短日照诱导下的变化却不同,冀红9218的SPS活性在3个时期较对照升高,而唐山红小豆却降低,这与品种的熟性有关;而谷草转氨酶(GOT)和谷丙转氨酶(GPT)的活性受短日照的影响不大。初花后短日照诱导效应较开花前减弱,但还具有一定的调控作用,小豆叶片内的生理参数通过短日照诱导而被削弱,进而影响小豆的生长发育。

In order to clarify the effects of short-day photoperiod inducement after early flowering on physiological parameters in adzuki bean, medium maturing variety Jihong 9218 and late maturing variety Tangshanhongxiaodou were treated by different short-day photoperiod at Early Flowering-Early Podding （EF-EP）, Early Podding-Early Seed （EP-ES）and Early Flowering-Early Seed （EF-ES）three stages, to investigate the effects of short-day photoperiod inducement on physiological parameters in adzuki bean leaves. Short-day photoperiod inducement decreased the chlorophyll content of two adzuki bean varieties, net photosynthetic rate also showed a decreasing trend at the same time.Which leads to decreased the maximum photochemical quantum yield （Fv/Fm）;Soluble sugar and soluble protein content of two adzuki bean varieties were decreased by short-day photoperiod inducement after early flowering, and 12 h short-day photoperiod inducement had a greater influence at the EF-ES stage, and then leading to slower carbon and nitrogen metabolism, meanwhile, sucrose synthetase （SS）activity showed a decreasing trend, it was thus clear that the rate of carbon metabolism was related to SS activity, but the activities of sucrose phosphate synthase （SPS）in two varieties were different, sucrose phosphate synthase （SPS）activity of Jihong 9218 increased at three stages compared with CK while Tangshanhongxiaodou decreased, which might be related to the ripening of varieties;glutamic-oxalacetic transaminase （GOT）and glutamic-pyruvic transaminase （GPT）activity were not affected by photoperiod. The induction effect of short-day induction effect after anthesis was weaker than that before anthesis, but it also had a certain control effect, physiological parameter in adzuki bean could be regulated by short-day photoperiod inducement after early flowering, thereby affecting the growth and development of adzuki bean.