血清IL-8、Fractalkine、MIP-3α对于初诊肺癌患者病情的诊断价值

Diagnostic value of serum IL-8,Fractalkine and MIP-3α on state of illness in patients with first diagnosed lung cancer

目的探讨白细胞介素-8(IL-8)、Fractalkine和巨噬细胞炎性蛋白-3α(MIP-3α)对于初诊肺癌患者病情的诊断价值。方法选择279例肺癌患者及同期进行体检的志愿者70例的资料进行回顾性分析。首先按照TNM分期将患者分为Ⅰ期组72例、Ⅱ期组73例、Ⅲ期组75例、Ⅳ期组59例。同时将初诊肺癌患者按照病理类型分为小细胞癌组91例与非小细胞癌组188例。分析各组患者IL-8、Fractalkine、MIP-3α指标及其之间的关系。结果按照TNM分期分组的情况下,五组患者的血清IL-8、Fractalkine和MIP-3α浓度比较,差异均有统计学意义(F分别=23749.66、10655.44、3959.77,P均<0.05)。两两比较显示,TNM不同分期组患者的IL-8、Fractalkine和MIP-3α浓度均明显高于对照组(q分别=8.53、7.40、6.55、5.66、11.26、12.36、10.29、8.88、7.65、5.70、6.67、9.92,P均<0.05),且IL-8、Fractalkine和MIP-3α浓度与TNM分期呈正相关(r分别=0.98、0.98、0.97,P均<0.05)。按照病理类型分组的情况下,三组患者的血清IL-8、Fractalkine和MIP-3α浓度比较,差异均有统计学意义(F分别=345.27、478.91、447.58,P均<0.05)。两两比较显示,小细胞癌组和非小细胞癌组患者的IL-8、Fractalkine和MIP-3α浓度均明显高于对照组(q分别=7.72、6.46、8.92、9.55、11.25、9.57,P均<0.05),但小细胞癌组和非小细胞癌组之间比较,差异无统计学意义(q分别=1.20、0.22、0.22,P均>0.05)。利用血清IL-8、Fractalkine和MIP-3α浓度指标对五组患者进行判别分析,交叉验证判别正确率为100%。结论初诊的肺癌患者IL-8、Fractalkine和MIP-3α三种趋化因子的浓度水平高于健康人,并且与疾病严重程度相关,三种趋化因子的浓度与肺癌类型并没有关系,利用三种趋化因子水平对初诊肺癌患者进行病情推断拥有较高的诊断价值。

Objective To investigate the diagnostic value of interleukin-8（IL-8）,fractalkine and macrophage inflammatory protein-3α（MIP-3α）on state of illness in patients with first diagnosed lung cancer. Methods The retrospective analysis was performed on 279 patients with lung cancer and 70 volunteers who underwent physical examination during the same period. First,according to TNM staging,the patients were divided into 72 patients in stage I,73 in stage Ⅱ,75 in stage Ⅲ,and 59 in stage Ⅳ. At the same time,the first diagnosed lung cancer patients were divided into small cell cancer group（91 cases）and non-small cell cancer group（188 cases）according to pathological types. The concentrations of IL-8,Fractalkine,MIP-3α indicators and their relationship among groups were analyzed. Result The differences in serum IL-8,Fractalkine and MIP-3α concentrations among groups with different TNM stage were statistically significant（F=23749.66,10655.44,3959.77,P〈0.05）. The concentrations of IL-8,Fractalkine and MIP-3αin the different TNM stage groups were significantly higher than those of the control group（q=8.53,7.40,6.55,5.66,11.26,12.36,10.29,8.88,7.65,5.70,6.67,9.92,P〈0.05）. The concentrations of IL-8,Fractalkine and MIP-3α were positively related with TNM stage（r=0.98,0.98,0.97,P〈0.05）. The serum IL-8,Fractalkine and MIP-3α concentrations among the groups with different pathological type were statistically significant（F=345.27,478.91,447.58,P〈0.05）. The IL-8,Fractalkine and MIP-3α concentrations of the small cell carcinoma group and the non small cell carcinoma group were significantly higher than those of the control group（q=7.72,6.46,8.92,9.55,11.25,9.57,P〈0.05）. However,there were no significant differences in the IL-8,Fractalkine and MIP-3α concentrations between the small cell carcinoma group and the non small cell cancer group（q=1.20,0.22,0.22,P〉0.05）.The serum levels of IL-8,Fractalkine and MIP-3α were used to discriminate the severity of the disease. The accuracy rate of cross validation was 100%.Conclusion The levels of IL-8,Fractalkine and MIP-3αof the primary lung cancer patients were higher than those of healthy people. And the concentration of the three chemokines were positively relate to the severity of the disease,but were not related to the type of lung cancer. The concentration of the three chemokines have high value for predict the severity of lung cancer.