HPLC测定阿齐沙坦片的含量

Determination of Azilsartan Tablets by HPLC

目的建立高效液相色谱法(HPLC)测定阿齐沙坦片含量的方法。方法采用Welch Ultimate XB C18(150 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-缓冲液(p H 2.8)(取磷酸二氢钠1.56 g和戊烷磺酸钠0.87g,加入1000 ml水使溶解,用磷酸调pH 2.8)(45:55),检测波长251 nm,流速1.0 ml/min。结果阿齐沙坦浓度在0.021~0.5232 mg/ml范围内线性关系良好(r=1.0000),平均回收率为100.15%,RSD为0.65%。结论本方法专属强、准确度高,可用于阿齐沙坦片的含量测定。

Objective To establish an HPLC method for the determination of Azilsartan Tablets. Methods A stable HPLC method was established, and the chromatography was accomplished on an Welch Ultimate XB C18 column （150 mm× 4.6 mm, 5 μm）, the mobile phase consisted of acetonitrile-pH 2.8 buffer （1.56 g of sodium dihydrogen phosphate and 0.87 g of sodium pentane sulfonate were dissolved in 1000 ml of water, and the pH was adjusted to 2.8 with phosphoric acid） （45：55）, the detection wavelength was 251 nm, and the flow rate was 1.0 ml/min. Results The calibration curve was linear in the range of 0.021-0.5232 mg/ml （r=1）, the average recovery was 100.15 %, with corresponding RSD of 0.65 %. Conclusion This method has high specialty and accuracy, and can be used for the determination of Azilsartan Tablets.