炎症状态下牙周膜干细胞生物学行为的变化

The biological characteristics of periodontal ligament stem cells in inflammatory microenvironment

目的:探讨慢性牙周炎症状态下牙周膜干细胞(PDLSCs)生物学行为的变化。方法:通过有限稀释法从正常和炎症牙周组织中分离、培养牙周膜干细胞,流式细胞仪鉴定细胞表型;细胞周期检测、MTT分析和克隆形成率检测细胞增殖能力;成骨、成脂诱导检测细胞分化能力。结果:在炎症感染的牙周组织中同样含有牙周膜干细胞,两种PDLSCs的细胞表面分子表达基本一致。流式细胞仪检测两种PDLSCs均表达间充质干细胞的表面标志物,且表达一致;与来自健康牙周组织的PDLSCs相比,来自牙周炎症组织的PDLSCs增殖能力增强,但成骨、成脂分化能力减弱。结论:在慢性炎症微环境的作用下,PDLSCs的生物学行为发生改变,增殖能力增强,而分化能力减弱。

Objective： To investigate the biological chatacteristics of periodontal ligament stem cells （PDLSCs） in inflammation microenvironment. Methods： PDLSCs were isolated from the periodontal ligament tissues of periodontitis patients （P-PDLSCs） and healthy controls （H-PDLSCs） by limiting dilution technique. Cell phenotype was analyzed by flow cytometry. Cell cycle analysis, MTT viability assay and clone-forming ability were used to study the proliferative ability. Osteogenic and adipogenic induction were performed to study the multipotential differentiation. Results： PDLSCs were successfully isolated from periodontitis patients and healthy controls. Both H-PDLSCs and P-PDLSCs expressed stem cell markers of mesenchymal stem cells and the overall expression patterns were similar between two groups. The proliferative ability of P-PDLSCs was strikingly higher than that of H-PDLSCs. The osteogenic and adipogenic ability of P-PDLSCs was lower than that of H-PDLSCs. Conclusions： In inflammatory microenvironment, PDLSCs exhibited increased proliferative ability but impaired differentiation capacity.