2017～2018年成都市三区县外环境禽流感监测分析

Surveillance and Analysis of Avian Influenza in the External Environment of three districts in Chengdu City from 2017-2018

目的监测四川省成都市三区县外环境中禽类流行性感冒(禽流感)病毒的分布状况,为人禽流感防控提供参考依据。方法我中心在2017年5月~2018年5月采用实时荧光定量PCR检测成都市三区县外环境中甲型流感通用病毒,再将阳性标本进行H5、H7、N9和H9分型,采用χ2检验比较定性资料。结果在497份样本中,甲型流感病毒阳性标本87份,检出率为17.91%;分型实验中,H5、H7、N9和H9分别检出7份、6份、12份和41份,其阳性检出率依次为1.41%、1.21%、2.41%、8.25%,未分型成功23份,占总样本的4.63%。结论成都市三区县外环境存在禽流感病毒污染,有人感染禽流感风险;需加强对外环境及高危人群禽流感监测,并规范对市场的管理机制,落实消毒和检疫准入等预防措施。

Objective To monitor the distribution of avian influenza（avian influenza）virus in the external environment the three districts of Chengdu,Sichuan province,and provide reference for the prevention and control of human avian influenza.Methods From May 2017 to May 2018,our center used real-time fluorescent quantitative PCR to detect influenza A virus in the external environment of the three districts of Chengdu.The positive samples were classified into H5,H7,N9 and H9,qualitative data were compared using the χ2 test.Results Among the 497 samples,87 were positive for influenza A virus,and the detection rate was 17.91%.In the typing experiment,7,6,12 and 41 were detected for H5,H7,N9 and H9,respectively.The positive detection rate was 1.41%,1.21%,2.41%,and 8.25%,and 23 cases were untyped,accounting for 4.63% of the total sample.Conclusion There is avian influenza virus contamination in the external environment the three districts of Chengdu,and some people are infected with avian influenza.It is necessary to strengthen the monitoring of avian influenza in the external environment and high-risk population,and regulate the market management mechanism and implement preventive measures such as disinfection and quarantine access.