尿液HBP,LE,U-NIT和WBC联合检测与尿液细菌定量培养对急性尿道感染的诊断意义

Diagnostic Significance of Urine HBP,LE,U-NIT and WBC Combined with Urine Bacterial Quantitative Culture in the Diagnosis of Acute Urethral Infection

目的探讨尿液肝素结合蛋白(HBP)、白细胞酯酶(LE)、尿亚硝酸盐(U-NIT)和白细胞计数(WBC)联合检测与尿液细菌定量培养对急性尿道感染的诊断意义。方法收集2017年1～12月在黄石市中心医院确诊的98例急性尿道感染患者为病例组(诊断结果以患者出院时诊断为准),选取同年龄段、同时间段来医院体检的健康患者95例作为对照组,分别在不同时间段留取对照组、治疗前病例组和治疗后病例组患者尿液标本10ml于无菌塑料管中(治疗前病例组所有病例均为患者入院还未进行任何治疗,治疗后病例组所有病例均为患者入院后接受治疗病情已基本稳定),留取的尿液标本均在1h内进行HBP,LE,U-NIT和WBC检测及细菌定量接种,对动态监测的结果进行统计学分析。结果治疗前病例组与对照组比较,差异有统计学意义(P<0.01);治疗后病例组与治疗前病例组比较,差异均有统计学意义(P<0.01);五项联合检测在不同时间段敏感度、特异度和准确度均高于单项检测及四项联合检测,差异均有统计学意义(P<0.01),约登指数(敏感度+特异度-1)最高为0.81。结论尿液HBP,LE,U-NIT和WBC联合检测与尿液细菌定量培养对急性尿道感染快速诊断具有很高的临床价值。

Objective To explore the diagnostic significance of urine HBP, LE, U-NIT and WBC combined with urine bacterial culture in the diagnosis of acute urethral infection. Methods 98 cases of acute urinary tract infection were collected as the case group in Huangshi City Central Hospital ftom January to December 2017 ,and selected 95 healthy patients of the same age group and the same time period who came to the hospital for physical examination as the control group. 10 ml urine samples ftom patients in the conteol group and the case group were collected at different time periods in sterile plastic tubes. All urine samples were tested for HBP, LE,U-NIT,WBC and Quantiative inoculation of bacteria within 1 h, and the results of dynamic monitoring were statistically analyzed. Results Compared with the control group before treatment,the level of five indicators was significantly higher,and the difference was statistically significant f P〈0.01）. Compared with the pre-treatment group, the level of the five indicators decreased significantly, and the difference was statistically significant f P〈0.01）. Five joint detection sensitivity at different times, specific degrees and accuracy were higher than single detection and four joint detection,the difference was statistically significant f P〈0.01）, some index fsensitivity~ specificity-1） up to 0.81. Conclusion Urine HBP, LE, U-NIT,WBC and urine bacterial quantitative culture have high clinical value for rapid diagnosis of acute urethral infection.