人脑胶质瘤组织中趋化因子受体和血管内皮细胞生长因子的表达

Expression of CXCR7 and VEGF in Gliomas of Human

目的:探讨趋化因子受体(CXCR7)和血管内皮细胞生长因子(VEGF)在胶质瘤患者肿瘤组织的表达及其与胶质瘤发生发展的关系。方法:应用组织芯片技术及免疫组织化学SP法,检测10例正常脑组织和69例胶质瘤组织CXCR7和VEGF的表达水平,比较不同病理分级胶质瘤组织中CXCR7和VEGF的表达水平,采用Spearman法分析胶质瘤组织中CXCR7和VEGF的相关性。结果:正常脑组织(对照组)、低级别胶质瘤组及高级别胶质瘤组患者脑组织中CXCR7、VEGF的表达阳性率逐渐升高,组间比较差异有统计学意义(P <0. 05);CXCR7、VEGF表达随胶质瘤恶性程度的增加表达逐渐上调,呈正相关(r=0. 327,P <0. 01); Spearman等级分析显示,CXCR7与VEGF在69例胶质瘤组织中的表达呈正相关关系(r=0. 327,P <0. 01)。结论:CXCR7及VEGF在胶质瘤组织中表达上调,且随着肿瘤恶性程度的增高而增强,CXCR7和VEGF在胶质瘤的发生、发展过程中可能起协同作用。

Objective：To investigate the expression of CXCR7 and VEGF in tissue of gliomas,and the relationship between CXCR7,VEGF and pathologic grading as well as tumorigenesis of gliomas. Methods： Immunohistochemistry （SP） tissue chip were applied to evaluate the expression of CXCR7 and VEGF in 10 cases of normal controls and 69 cases of human gliomas; comparing expression of CXCR7 and VEGF in different pathological gradings of gliomas; adopting Spearman assay to analyze relevance of CXCR7 and VEGF in gliomas tissue. Results： The positive expression rates of CXCR7 and VEGF gradually increased in healthy brain tissue of control group, low grade gliomas group and high grade gliomas group, comparison between groups was statistical significant （ P 〈0.05）. The expression of CXCR7 and VEGF upregulated with the increase of malignant level of gliomas, which was positively correlated （ r=0.327,P〈 0.01）. Spearman grading analysis indicated that expression of CXCR7 and VEGF was positively correlated in 69 cases of gliomas tissue （ r=0.327,P〈 0.01）. Conclusion： The expression of CXCR7 and VEGF in gliomas was upregulated and the positive reaction rate of CXCR7 and VEGF were increased with the increase of tumor malignancy. The results indicate CXCR7 and VEGF may play a co-ordinating role in the tumorigenesis of gliomas.