Slug过表达对口腔鳞癌Tca8113细胞侵袭性的影响

Overexpression of Slug promotes invasiveness of oral squamous cell carcinoma Tca8113 cell line

目的:研究Slug过表达对口腔鳞癌Tca8113细胞侵袭性的影响。方法:将Tca8113细胞培养于含有10%血清的1640培养基中,构建带有使Slug过表达的质粒GV141/Slug和空载体的质粒GV141,分别转染口腔鳞癌Tca8113细胞,并设置空白对照组。通过观察荧光和采用qPCR和Western blot检测Slug的表达来观察转染效率。qPCR和Western blot检测3组细胞中的E-钙黏蛋白(E-cadherin)和波形蛋白(Vimentin)的表达变化,划痕实验检测细胞转移能力的区别,Transwell实验检验细胞侵袭能力的变化。结果:Slug过表达质粒转染效率为50%~60%,GV141/Slug组的Slug和Vimentin mRNA和蛋白表达水平明显高于其余2组,而E-cadherin的蛋白表达量显著降低,划痕实验和Transwell实验表明GV141/Slug细胞的转移和侵袭能力明显强于其余2组(均P<0.05)。结论:Slug过表达可通过减少E-cadherin的含量而促进口腔鳞癌Tca8113细胞的转移。

Objective： To investigate the effect of Slug overexpression on metastasis of oral squamous cell carcinoma （OSCC） cell line Tca8113. Methods： Tca8113 was cultured in RIPM1640 with 10% FBS. Plasmids encoding Slug mRNA and empty plasmid were transfected to Tca8113 respectively, the transfection efficiency was examined by observing fluorecene and detecting the change of Slug expression by qPCR and Western blot assay, qPCR and Western blot assay detected changes in the expression of E-cadherin and Vimentin in three groups of cells. The scratch experiment detected the difference in cell metastasis. The Transwell experiment examined the changes in cell invasion ability. Results： Transfection efficiency was about 50%-60%, the mRNA and protein from Slug and Vimentin in GV141/slug group was obviously higher than the other two groups （P〈0.05）. While E-cadherin expression was significantly decreased （P〈0.05）. Wound healing and Transwell assays indicated that the ability of invation in GV141/slug cells was stronger than that of the other two groups （P〈0.05）. Conclusion： In this study, we found Slug expression could increased the ability of invation of Tca8113 cells by regulating EMT.