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Screening of Actinomycetes from Medicinal Plant Rhizosphere Soils for Industrial Enzymes and Antimicrobial Activity

Screening of Actinomycetes from Medicinal Plant Rhizosphere Soils for Industrial Enzymes and Antimicrobial Activity
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摘要 This study aimed to investigate the production of some metabolites (i.e., antibiotics, amylases and cellulases) of terrestrial actinomycetes isolated from medicinal plant rhizosphere soils. Initially, the soil samples were collected from Camellia sinensis (L) Okuntze., Peuraria mirifca Airy Shaw Suvatabandhua., Ananus comosus Merr., Elephantopus scaber Linn., Orthosiphon grandiforus Bolding., Jatropha multifda Linn. and Senna siamea. To screen and isolate actinomycetes, the soil samples were pretreated by air-drying and subsequent heat incubation. The bacterial isolates exhibiting actinomycetes features were then randomly screened for their production of amylases, cellulases and antibiotics. It was found that 130 isolates (from 136) could produce amylases; 40 (from 107) produced cellulases; and seven (from 45) exhibited antimicrobial activity. The data of this study were preliminary, and yet demonstrated a rich diversity of rhizo-actinomycetes from medicinal plants. Besides, these organisms could be an untapped source for discovering of biotechnologically useful metabolites. This study aimed to investigate the production of some metabolites (i.e., antibiotics, amylases and cellulases) of terrestrial actinomycetes isolated from medicinal plant rhizosphere soils. Initially, the soil samples were collected from Camellia sinensis (L) Okuntze., Peuraria mirifca Airy Shaw Suvatabandhua., Ananus comosus Merr., Elephantopus scaber Linn., Orthosiphon grandiforus Bolding., Jatropha multifda Linn. and Senna siamea. To screen and isolate actinomycetes, the soil samples were pretreated by air-drying and subsequent heat incubation. The bacterial isolates exhibiting actinomycetes features were then randomly screened for their production of amylases, cellulases and antibiotics. It was found that 130 isolates (from 136) could produce amylases; 40 (from 107) produced cellulases; and seven (from 45) exhibited antimicrobial activity. The data of this study were preliminary, and yet demonstrated a rich diversity of rhizo-actinomycetes from medicinal plants. Besides, these organisms could be an untapped source for discovering of biotechnologically useful metabolites.
机构地区 School of Science
出处 《Journal of Northeast Agricultural University(English Edition)》 CAS 2018年第3期23-28,共6页 东北农业大学学报(英文版)
关键词 ACTINOMYCETE AMYLASE antibiotic CELLULOSE rhizosphere soil actinomycete amylase antibiotic cellulose rhizosphere soil
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