分子标记辅助聚合基因Pi1和Pi2改良红色特种籼稻稻瘟病抗性研究

Improvement of Rice Blast Resistance in Red Rice by Pyramiding of Pi1 and Pi2 through Molecular Marker-Assisted Selection

红色特种籼稻是一种极其珍贵的特色稻米资源,稻瘟病是红色特种籼稻的主要病害,培育抗稻瘟病品种是防治稻瘟病的有效途径。以含广谱稻瘟病抗性基因Pi1和Pi2的BL122为供体,红色特种籼稻桂红一号为受体,通过杂交、回交和自交并结合分子标记辅助选择,将Pi1和Pi2基因导入红色特种籼稻桂红一号中,获得了携带2个抗性基因的高抗稻瘟病改良材料。对BC3F3代材料进行了苗期和成熟期稻瘟病抗性鉴定,结果表明,携带2个抗性基因的目标株系抗性均达到抗以上水平,部分株系达到高抗水平,稻瘟病抗性显著高于轮回亲本。统计分析表明,BC3F3改良材料与对照相比,有效穗数和理论产量显著增加,而在其他农艺性状方面无显著差异。通过分子标记辅助选择获得的改良材料为红色特种籼稻的抗稻瘟病育种创制了新的种质资源。

Red rice is an extremely precious characteristic rice resource. Blast is one of the most serious rice diseases worldwide,which usually leads to a shaqo decline in rice yield and even results in no yield. Breeding blast-resistant varieties is one of the most effective and economical ways to control the disease. In this study,BL122 containing two blast resistance genes P/1 and P/2 was used as gene donor to be crossed and then backcrossed with red rice variety Guihong 1. The improved materials of high resistance to rice blast with two resistance genes were obtained. The resistance identification of rice blast in seedling stage and mature stage of BC3F3 was carried out. The results showed that the resistance of target strains with two resistance genes was all above the resistance level,and some strains reached high resistance level. The resistance of these strains to rice blast was significantly higher than that of the recurrent parent. Statistical analysis showed that,compared with the control,the effective panicle and theoretical yield of BC3F3 were significantly increased, while there was no significant difference in other agronomic traits. The improved materials obtained through molecular marker-assisted selection is a new breeding resource for the breeding of red rice.