摘要
Calcium （Ca2 ＋） signaling has been implicated in poJ]en germination and pollen tube growth. To date, however, we still know very little about how exactly Ca2＋ signaling links to various physiological subcellular processes during pollen germination and pollen tube growth. Given that Ca2＋ signaling is tightly related to the cytosolic concentration and dynamics of Ca2＋, it is vital to trace the dynamic changes in Ca＋ levels in order to decode Ca2＋ signaling. Here, we demonstrate that G-CaMP5 serves well as an indicator for monitoring cytosolic Ca2＋ dynamics in pollen cells. Using this probe, we show that cytosolic Ca2＋ changes dramatically during pollen germination, and, as reported previously, Ca2＋ forms a tip-focused gradient in the pollen tube and undergoes oscillation in the tip region during pollen tube growth. In particular, using G-CaMP5 allowed us to capture the dynamic changes in the cytosolic Ca2＋ concentration （[Ca2＋ ]cyt） in pollen tubes in response to various exogenous treatments. Our data suggest that G-CaMP5 is a suitable probe for monitoring the dynamics of [Ca2＋ ]cyt in pollen cells.
Calcium （Ca2 ＋） signaling has been implicated in poJ]en germination and pollen tube growth. To date, however, we still know very little about how exactly Ca2＋ signaling links to various physiological subcellular processes during pollen germination and pollen tube growth. Given that Ca2＋ signaling is tightly related to the cytosolic concentration and dynamics of Ca2＋, it is vital to trace the dynamic changes in Ca＋ levels in order to decode Ca2＋ signaling. Here, we demonstrate that G-CaMP5 serves well as an indicator for monitoring cytosolic Ca2＋ dynamics in pollen cells. Using this probe, we show that cytosolic Ca2＋ changes dramatically during pollen germination, and, as reported previously, Ca2＋ forms a tip-focused gradient in the pollen tube and undergoes oscillation in the tip region during pollen tube growth. In particular, using G-CaMP5 allowed us to capture the dynamic changes in the cytosolic Ca2＋ concentration （[Ca2＋ ]cyt） in pollen tubes in response to various exogenous treatments. Our data suggest that G-CaMP5 is a suitable probe for monitoring the dynamics of [Ca2＋ ]cyt in pollen cells.
基金
supported by grants from the National Natural Science Foundation of China(31471266 and 31671390)
supported by a post-doc fellowship from Tsinghua-Peking Joint Center for Life Sciences
