摘要
目的利用血管张力固定改良PE管,制作血栓栓塞大脑中动脉脑梗死(Embolic middle cerebral artery occlusion,eMCAO)大鼠模型。方法采用清洁级Sprague-Dawley大鼠20只制作eMCAO模型,均为单人制作,利用血管张力固定改良PE管。结果改良PE插管均成功,大鼠eMCAO造模成功率90%,制作时间每只平均30 min。结论利用血管张力法可单人操作制作大鼠eMCAO模型,有效节省造模所需时间以及人力物力。
Objective To improve the method of establishing embolic middle cerebral occlusion （eMCAO） rat model. Method Twenty Sprague-Dawley rats were used to establish the rat eMCAO model, the modified PE tube was fixed and inserted by using the vascular tone. Result The modified PE tube was all inserted, the average time of making the eMCAO model was 30 min, 90% of the rats was successfully made of eMCAO. Conclusion The vascular tone can be used in the model making of rat eMCAO with reducing the time and labor cost.
作者
刘晓静
蔡桂兰
毕鸿雁
陈旭
张拥波
LIU Xiaojing;CAI Guilan;BI Hongyan;CHEN Xu;ZHANG Yongbo(Department of Neurology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)
出处
《实验动物科学》
2018年第4期37-39,44,共4页
Laboratory Animal Science
基金
国家自然科学基金(No.81671191)
北京市卫生人才系统高层次卫生技术人才学科骨干基金(No.2015-3-001)
