SCID小鼠脏器质量和部分血液生理生化及免疫指标测定

Detection of Main Organ Weights, Blood Physiological,Biochemical and Immunologcal Indexes in SCID Mice

目的测定SCID小鼠主要脏器质量、血液生理生化、免疫球蛋白、补体及免疫细胞数据。方法选择4周龄、8周龄、16周龄的SCID小鼠20只(雌雄各半),测定主要脏器质量、血液生理生化、免疫球蛋白、补体数据;选择8周龄的SCID小鼠20只(雌雄各半),测定T细胞及亚群、B细胞、NK细胞免疫指标。结果 SCID小鼠的胸腺质量与性别、周龄均无关,各数据间无显著差异。4周龄SCID小鼠的红细胞(RBC)、淋巴细胞(LYM)、免疫球蛋白G(IgG)、免疫球蛋白A(IgA)、免疫球蛋白M(IgM)水平显著大于8周龄、16周龄的SCID的小鼠。用脾脏采样分离的T细胞、B细胞、NK细胞在性别上无显著差异,CD3+在整体免疫细胞占比最小,B220+占比最大。结论 SCID小鼠表现为T、B细胞免疫缺陷,本实验测定的生理生化数据与国外SCID小鼠数据基本符合,表明该品系自引种以来未发生生物学特性明显改变。

Objective To determinate the main organ weights, blood physiological and biochemical indexes, immunoglobulins, complements and immune cells data of SCID mice. Methods Twenty SCID mice at the age of 4, 8 and 16 weeks old respectively, with half male and female, were selected for detecting the main organ weight, blood physiology and biochemistry indexes, immunoglobulins and complements data. Twenty SCID mice at the age of 8 weeks old, with half male and female, were selected for detecting T cells and subgroups, B cells, and NK cells. Results The thymus weight of SCID mice was independent of gender and age, and there was no significant difference. The levels of red blood cell, lymphocyte, Ig G, Ig A and Ig M in the 4-week-old SCID mice were significantly greater than those in 8 weeks and 16 weeks. T cells, B cells, and NK cells, separated and sampled from spleen, are no significant difference in gender. CD3＋ was the smallest proportion in total immune cells, and B220＋accounted for the largest proportion. Conclusions SCID mice are immunodeficiency animals of T and B cells comprised. The blood physiological and biochemical data of this experiment were basically consistent with the data of SCID mice abroad. It shows that the biological characteristics, such as heredity character, physiology biochemistry of this strain have not changed since the introduction.