基于药效评价的主成分分析法对不同规格鹿茸差异性研究

Studies on Effects of Different Cornu Cervi Pantotrichum on Bone Marrow Mesenchymal Stem Cells Based on Principal Component Analysis

目的考察8种不同规格鹿茸含药血清对骨髓间质干细胞(BMSCs)增殖及成骨分化的影响,并对8种不同规格鹿茸进行主成分分析(PCA),揭示不同规格鹿茸的相似性和差异性。方法雌性SD大鼠72只,随机分为9组,按鹿角的生长特点、加工方式等筛选8种不同规格的鹿茸药材,分别制得不同规格鹿茸的大鼠含药血清,以上述血清对BMSCs细胞进行培养,MTT法检测成骨细胞的增殖情况,ELISA法检测碱性磷酸酶(ALP)、骨形成蛋白2(BMP-2)、骨钙素(BGP)分泌量,以测定结果作为PCA数据源进行统计分析。结果吸光度(A)值,ALP值的PCA能将鹿茸蜡片与骨片、血片区别开来,正确率为100%。结论本方法及模型可用于鹿茸药材质量评价。

Objective To systematically evaluate the similarity and discrepancy of eight different Cornu Cervi Pantotrichum by principal component analysis（PCA） which could accelerate the proliferation of the bone marrow mesenchymal stem cells cultured in vitro and their differentiation toward osteoblasts.Methods Seventy-two female SD rats were divided into nine groups randomly, one normal group given water and eight administrated group. Cornu Cervi Pantotrichum was classified by growth and processes.Bone marrow mesenchymal stem cells（BMSCs） were exposed to different culture medium containing different Cornu Cervi Pantotrichum serum. The activities of osteoblastic markers such as alkaline phosphatase（ALP）, bone gla protein（BGP）, bone morphogenetic protein 2（BMP-2） were detected by ELISA.Cell proliferation was measured by MTT assay. The principal component analysis was applied to study characteristic of velvet antler based on the indexes mentioned above. Results PCA analysis of absorbance value（A） and ALP can distinguish wax slices from blood slices and bone slices. The accuracy of the discriminant analysis was 100% for A and ALP. Conclusion This method can be used to evaluate the quality of Cornu Cervi Pantotrichum.