浸出芝麻油中芝麻木酚素提取工艺的研究

Extraction of sesame lignan from leaching sesame oil

以浸出芝麻毛油为原料,乙醇为提取剂,进行芝麻木酚素的提取。通过单因素试验和正交试验确定了最佳的芝麻素提取工艺条件,并利用高相液相色谱仪对得到的芝麻木酚素粗品进行芝麻素和芝麻林素含量测定。结果表明:芝麻素提取工艺的最佳条件为浸提次数3次、料液比1∶4、提取温度60℃、乙醇体积分数95%、搅拌时间0.5 h、静置时间2 h、搅拌速度400 r/min,在此条件下芝麻素提取率为86.99%;得到的芝麻木酚素粗品中芝麻素含量为48.73%,芝麻林素含量为5.71%。

With leaching crude sesame oil as raw material and ethanol as extractant, sesame lignan was extracted. The optimal extraction conditions of sesamin were obtained by single factor experiment and orthogonal experiment. The contents of sesamin and sesamolin in crude sesame lignan were determined by HPLC. The results showed that the optimal extraction conditions were obtained as follows： leaching times three, solid - liquid ratio 1 ： 4, extraction temperature 60 ℃, volume fraction of ethanol 95%, stirring time 0.5 h, standing time 2 h and stirring speed g00 r/min. Under these conditions, the extraction rate of sesamin was 86. 99%, and the contents of sesamin and sesamolin in crude sesame lignan were 48.73 % and 5.71% respectively.