大鲵油体外抗氧化活性研究

In vitro antioxidant activity of Chinese giant salamander oil

采用体外抗氧化实验研究大鲵油抗氧化活性,测定大鲵油对DPPH自由基、羟基自由基、超氧自由基清除能力和还原能力、Fe^(2+)螯合能力。以V_C为阳性对照,评价大鲵油的体外抗氧化活性。结果表明:大鲵油抗氧化能力强于鲐鱼油,添加复合抗氧化剂后抗氧化能力更强,对Fe^(2+)的螯合能力表现最好,相同质量浓度条件下强于V_C;对DPPH自由基、羟基自由基清除能力和还原能力与V_C相当。大鲵油对DPPH自由基、羟基自由基和Fe^(2+)螯合能力的半抑制质量浓度(IC_(50))分别为3.523、0.493、0.559 mg/m L,添加了复合抗氧化剂的大鲵油的相应IC_(50)分别为0.572、0.099、0.062 mg/m L。

In order to investigate in vitro antioxidant activity of Chinese giant salamander oil, the antioxi- dant activity of Chinese giant salamander oil was evaluated by DPPH radical, hydroxyl radical, superox- ide anion radical scavenging ability assays, reducing capacity assay and capacity of chelating Fe2＋ with vitamin C as positive control. The results indicated that the antioxidant ability of Chinese giant salaman- der oil was stronger than mackerel oil, and the antioxidant ability was stronger after adding compound an- of DPPH radical, hydroxyl radical and reducing capacity. The IC50 values of Chinese giant salamander oil on DPPH radical, hydroxyl radical and capacity of chelating Fe2＋ were 3. 523,0. 493 mg/mL and 0. 559 mg/mL respectively, and they were 0. 572,0. 099 mg/mL and 0.062 mg/mL for Chinese giant salamanderoil added with compound antioxidant, respectively.