香椿种子无菌苗再生体系的建立

Establishment of rapid propagation for sterile seedlings of Toona sinensis by tissue culture

为了建立香椿无性系规模化组培快繁技术,以成熟香椿种子在无菌条件下萌发的幼苗茎段为外植体,研究不同基本培养基和不同植物生长调节剂对丛芽诱导、增殖、壮苗及生根的影响。结果表明:最佳消毒处理为20%NaClO消毒20 min,萌发率为80.56%,污染率为27.27%;萌发培养基为WPM或1/2MS+3.0 mg·L^(-1)GA_3;最佳丛芽诱导培养基为MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA+1.0 mg·L^(-1)GA_3,增殖倍数为4.82;最佳壮苗培养基MS+0.5 mg·L^(-1)6-BA+0.05 mg·L^(-1)IAA+2.0 mg·L^(-1)GA_3;最佳生根培养基MS+2.0 mg·L^(-1)IBA,生根率为78.16%,平均生根条数为4.8。炼苗后,移栽到园土∶草炭土∶珍珠岩体积比为2∶2∶1的基质中,成活率达77.5%。本研究为香椿良种快繁提供了一条新途径,并较其他外植体(叶片、带芽茎段)为起始材料有不受取材条件限制的优点。

In order to establish the tissue culture and rapid propagation system of Toona sinensis. In this study, the seedlings of mature Toona sinensis seed germination under sterile conditions were used as explants to study the influence of different medium and different plant growth regulators on bud induction, hyperplasia, sound seedling and rhizogenesis and establish a complete system of tissue culture and rapid propagation. The results showed that the best disinfection treatment was 20% NaCIO disinfection for 20 min, the germination rate was 80.56%, the contamination rate was 27.27%. The best germination medium was 1/2MS＋3.0 mg·L-1GA3, and the best medium for bud induction was MS＋1.0 mg·L-16-BA＋0.1 mg·L-1NAA＋1.0 mg·L-1GA3, the proliferation times was 4.82.The best Seedling medium was MS＋0.5 mg·L-16-BA＋0.05 mg·L-1IAA＋2.0 mg·L-1GA3. The best medium for rooting was MS＋2.0 mg·L-1IBA, the rooting rate was 78. 16%, and the average number of rooting shoots was 4.8. The survival rate was 77.5% in the medium of 2：2：1 in peat soil： peat soil： perlite.The research supplied a new way for rapid propagation of Toona sinensis.