电针俞募穴对肠动力亢进大鼠海马、肠道双位点脑源性神经营养因子蛋白表达的影响

Effects of Electroacupuncture at Back-shu and Front-mu Points on Expression of BDNF in Hippocampal and Intestinal Tissue of Intestinal Hypermotility Rats

目的：探讨针刺治疗腹泻型功能性肠病的作用机制。方法：50只大鼠随机分为空白组、模型组、俞募配穴组、俞穴组、募穴组,每组10只。采用番泻叶煎剂灌胃法造模,同日电针刺激天枢、大肠俞,每次20 min,共治疗10次。运用光镜、HE染色、免疫组化等技术,观察治疗后大鼠排便情况、海马及肠道内BDNF蛋白表达水平。结果：模型组大鼠1 h排便粪粒数、24 h排便粪粒数、24 h粪便含水量和空肠BDNF蛋白含量均显著增高,海马DG区BDNF蛋白含量显著减少;针刺组能不同程度减少1 h排便粪粒数、24 h排便粪粒数,降低24 h粪便含水量、空肠BDNF蛋白含量,提高海马DG区蛋白表达水平,由以募穴组、俞募组明显。结论：针刺可以改善肠动力亢进大鼠的异常排便情况,通过影响空肠、海马DG区BDNF蛋白含量而发挥外周和中枢调节作用可能是其起效的一个重要机制。

Objective： To explore the mechanism of electroacupuncture at Back-shu and Front-mu points for intestinal hypermotility treatments. Methods： Fifty rats were randomly divided into control group,model group,Shu-Mu（ SM）points group,Back-Shu（ BS） points group,Front-Mu（ FM） points group,20 min each time for a total of 10 times treatment. By light microscopy,immunohistochemistry,H. E. Staining,the morphology of hippocampal and intestianl tissue structure were observed,and the levels of BDNF protein in hippocampe were detected. Restuls： The fecal pellet output,fecal water content and BDNF protein in jejunum were significantly increased in the model group than those in the control group（ P 〈0. 01）,and down-regulated in FM group and SM group（ P〈 0. 01）. The expression of BDNF protein in hippocampe in model groups was significantly decreased（ P 〈0. 01）. SM group,BS group and FM groups＇ expression of BDNF protein improved,especially in DG area（ P〈 0. 01）. Conclusion： Shu-Mu and Front-Mu points of large intestine meridians can better improve intestinal motor function of rats with hypermotility,which may be related to its effects in increasing the level of BDNF in hippocampal DG area tissue.