两种植物发酵液对氢化可的松致免疫抑制小鼠的免疫调节作用

Immunomodulatory Effect of Two Plant Fermentation Extracts(PFE)on Immunosuppressional Model Mice Induced by Hydrocortisone

目的:研究植物发酵液对氢化可的松致免疫抑制模型小鼠机体免疫功能的影响。方法:将健康雄性BALB/c小鼠随机分为正常组、模型组、莲博士组和古元真组,分别经口给予生理盐水或2.5 mL/kg BW(相当于人推荐日服量的5倍)莲博士或古元真酵素,连续灌胃30 d。从第23天起,除正常组外,各组均皮下注射氢化可的松溶液,连续注射7 d。正常组注射乙醇-生理盐水溶液作为对照。末次注射后测定血清免疫因子、肠免疫因子和胸腺脾脏指数。结果:两种植物发酵液能明显提高小鼠胸腺指数和血清IL-6、TNF-α、IFN-γ水平,且莲博士组有高于古元真组的趋势。两种植物发酵液能显著提高小肠SIgA水平,而对脾脏指数、血清IgG和补体C3水平无显著影响。结论:未发现两种植物发酵液显著改善细胞免疫状态,而能显著提高血清IL-6、TNF-α、IFN-γ水平,改善肠道免疫屏障,具有免疫促进功能。

Object： Effect of two plant fermentation extracts （PFE） on immunosuppressional mice induced by hydro- cortisone was investigated in this study. Method： Male BALB/c mice were randomly divided into 4 groups （including nor- mal, model, DrLeanne PFE and Yesdetox PFE） and orally administered the 2 PFEs respectively at a daily dosages of 2.50 mL/kg BW （comparatively as 5 times of recommended human daily dosage）. Saline was given to the normal group as the blank control. The treatments lasted for 30 days, and at the 23rd day, all the animals except the normal group were given hydrocortisone subcutaneously for 7 days, while the normal group was given ethanol-saline solution. At the 31~ day, all the animals were executed and indexes, including serum immune factors, intestinal immune factors and immune organ indexes, were measured. Compared with the model group, both PFEs significantly increased the thymus index as well as the contents of serum IL-6, TNF-α, IFN-γ No significant differences were observed in the spleen index, serum IgG contents and Complement 3 contents. The results indicate that the PFEs had no obvious impact on cellular immuni- ty, but enhanced the intestinal immune barrier, and significantly promoted serum IL-6, TNF-α, IFN-γlevels.