对虾中隐蔽态T-2毒素残留对雄性小鼠生长发育的影响及其遗传毒性效应

Effect of Masked T-2 Toxin in Litopenaeus vannamei on Growth and Development and Cytogenetic Toxicity of Male Mice

为探明对虾中隐蔽态T-2染毒对小鼠生长发育及遗传方面的毒性效应,采用定期递增剂量法进行20 d蓄积染毒实验,以对虾的虾头和肌肉匀浆液灌胃小鼠,连续7 d,记录小鼠器官指数、精子致畸率、微核率,分析对虾中T-2及隐蔽态残留物对小鼠的毒害作用。结果表明,低剂量染毒对虾对小鼠没有影响,高剂量组(12.2 mg/kg)染毒对虾引起小鼠增重率和器官系数下降,精子畸形率及微核率均显著高于对照组(P<0.05);随着染毒剂量的增加,精子畸形率及微核率呈上升趋势。染毒对虾中存在隐蔽态的T-2毒素,对小鼠有一定的遗传毒性,虾头中隐蔽态T-2毒素的含量高于肌肉中的,并与染毒剂量呈正相关。

Proven the toxic effect of masked T-2 toxin in Litopenaeus vannamei on growth and development and ge- netic of male mice. Mice, with head and muscle homogenates of the shrimps which were conducted the experiment of 20 d accumulation toxicity with Does-periodic-increased methods through tube feeding for 7 d. Then recording the organ index, spermatozoa deformity and micronucleus of marrow cell were measured. Analysis of the toxic effects of T-2 and the hidden residues in the shrimp on mice. The results showed that low doses T-2 infected shrimp has no effect on mice. However, high dose T-2 infected shrimp caused decreasing of organ index. Sperm deformity rate and micronucleus rate were significantly higher than that of the control group （P〈0.05）; With the increase of the exposure dose, the ab- normal rate of sperm and the rate of micronucleus increased. Research shows T-2 in feed could transformed into masked T-2 toxins （mT-2s） by shrimp after ingesting, T-2 was exposed to a certain genetic toxicity, and the contents of the masked T-2 in the head were higher than that in the muscle; which was positively correlated with the exposure dose.