自拟黄芪葛根汤合培元固本散对自身免疫性重症肌无力模型大鼠的影响

Study on the effect of Huangqi-Gegen decoction and Peiyuan-Guben powder on autoimmune myasthenia gravis rats

目的 研究自拟黄芪葛根汤合培元固本散对实验性自身免疫性重症肌无力（experimental autoimmune myasthenia gravis, EAMG）模型大鼠的影响及其作用机制。方法 将大鼠按随机数字表法分为空白组10只和造模组30只。造模组采用鼠源性乙酰胆碱受体a亚基97～116肽段（AChR-a97～116）免疫注射法进行EAMG大鼠建模。在第1次免疫后第11天进行第2次免疫。第1次免疫后第15天将大鼠按Lennon评分随机分为模型组、自拟黄芪葛根汤合培元固本散组（简称“黄培组”），泼尼松组，每组8只。黄培组大鼠灌胃黄芪葛根汤（21.5 g/kg）合培元固本散（0.8 g/kg），泼尼松组灌胃0.005 4 g/kg泼尼松水溶液，空白组和模型组灌胃等体积蒸馏水。按10 ml/kg大鼠体重给药，1次/d，共给药56 d。第1次免疫后，观察各组大鼠体重变化，采用Lennon评分法对各组大鼠进行评估。第1次免疫后第70天，检测腓肠肌电活动，计算波幅衰减程度。采用ELISA法检测血清中抗AChR-a97～116肽段抗体IgG及其亚型，检测血清中IL-4、IL-10、IL-17水平。结果 与模型组比较，第1次免疫第28天后，黄培组和泼尼松组大鼠体重增加（P＜0.05）。第1次免疫第36天后，黄培组大鼠Lennon评分降低（P＜0.05）。给药结束后，黄培组大鼠肌电图波幅衰减程度[（41.83±7.45）%比（67.76±4.32）%]降低（P＜0.05），大鼠血清中IgG[（1.15±0.07）比（1.24±0.08）]、IgG1[（0.17±0.01）比（0.25±0.03）]、IL-4[（16.54±1.66）pg/ml比（25.64±1.74）pg/ml]、IL-10[（113.65±12.87）pg/ml比（121.54±10.44）pg/ml]、IL-17[（43.58±3.54）pg/ml比（65.76±3.59）pg/ml]水平降低（P＜0.05）。结论 自拟黄芪葛根汤合培元固本散可增加大鼠体重，降低血清中AChR-Ab及IL-4、IL-10、IL-17水平，有效改善EAMG大鼠症状。

Objective Experimental model of experimental autoimmune Myasthenia Gravis （EAMG） were established to explore the effect of Zini-Huangqi-Gegen decoction and Peiyuan-Guben powder on EAMG rats model. Methods Experimental animals were randomly divided into the control group （n=10） and the model group （n=30）. The model rats were induced by murine AChR-α97-116 peptide immunostaining for EAMG rats model. After the first immunization, the general condictions and body weight of rats were observed, and the Lennon score was used to evaluate the rats. The second immunization was performed on the 11th day after the first immunization. On the 15th day after the first immunization, the rats were randomly divided into the model group（n=8）, Huangqi-Gegen decoction and Peiyuan-Guben powder group （abbreviated Huangpei group, n=8） and Prednisone group （n=8） according to the Lennon score. Huangpi group rats were treated with Huangqi-Gegen decoction （21.5 g/kg） combined with Peiyuan-Guben powder （0.8 g/kg）, prednisone group with 0.005 4 g/kg prednisone aqueous solution, the control group and the model group oral volume of distilled water. The rats were administered with a body weight of 10 ml/kg once a day for a total of 56 days. At the 70th day after the first immunization, serum was extracted from the rats. The Anti-AChR-α97-116 IgG and its subtype in serum were detected by ELISA. The IL-4, IL-10, IL-17 in serum were detected by ELISA. Results Compared with the model group, the weight of the rats in the Huangpei group and the prednisone group significantly increased after the 28th day of the first immunization （P〈0.05）. After the 36nd day of the first immunization, the Lennon score of the Huangpei group significantly decreased （P〈0.05）. At the end of the administration, the amplitude of EMG amplitude attenuation （41.83% ± 7.45% vs. 67.76% ± 4.32%） in the Huangpei group significantly decreased （P〈0.05）, and the serum IgG （1.15 ± 0.07 vs. 1.24 ± 0.08）, IgG1 （0.17 ± 0.01 vs. 0.25 ± 0.03）, IL-4 （16.54 ± 1.66 pg/ml vs. 25.64 ± 1.74 pg/ml）, IL-10 （ 113.65 ± 12.87 pg/ml vs. 121.54 ± 10.44 pg/ml）, IL-17 （43.58 ± 3.54 pg/ml vs. 65.76 ± 3.59 pg/ml） in the rat serum significantly decreased （P〈0.05）. Conclusions Huangqi-Gegen decoction and Peiyuan-Guben powder can increase the body weight of rats, decrease the concentration of AChR-Ab in serum, the concentrations of IL-4, IL-10 and IL-17 in serum, and effectively improve the symptoms of EAMG rats.