苏木对糖尿病大鼠血管内皮细胞自噬相关蛋白表达的影响

Effect of Sappan Wood on Expression of Autophagy Related Protein in Vascular Endothelial Cells of Diabetic Rats

目的探讨中药苏木通过调控BECN1、LC3-Ⅱ、p62自噬相关蛋白表达,对糖尿病大鼠血管内皮细胞的保护作用。方法将30只雄性SD大鼠随机分10只为正常组,其余20只进行糖尿病造模。成模后分为模型组与苏木组。苏木组给予810mg/kg每日1次灌胃。4周后,3组大鼠取主动脉,运用HE染色、透射电镜观察主动脉结构,Western-blot检测BECN1、LC3-Ⅱ、p62蛋白表达。结果苏木组血管平滑肌规则,厚薄均匀;透射电镜显示自噬体数量增多;Western-blot检测BECN1、LC3-Ⅱ的蛋白表达增加,p62的蛋白表达下降。结论苏木能调控自噬相关蛋白,保护糖尿病大鼠血管内皮的损伤。

Objective To investigate the protective effect of traditional Chinese medicine Sappan Wood on the vascular endothelium of diabetic rats by regulating the expression of BECN1, LC3- II and p62 protein. Methods 30 male SD rats were randomly into the normal group of 10 cases, and the remaining 20 were made mould for diabetes. The model was divided into the model module and the sappan wood group. The sappan wood group was given 810 mg/kg lavage once a day. After 4 weeks, the aorta was taken in three groups of rats. The aorta structure was observed by HE staining and transmission electron microscopy （TEM） . Results The vascular smooth muscle of the sappan wood group was uniform and thick. The transmission electron microscope showed that the number of autophagy and the protein expression of BECN1 and LC3- II increased, and the protein expression of p62 decreased. Conclusion The sappan wood can regulate autophagy, and protect vascular endothelial injury in diabetic rats.