Tissue Culture of Dianjiang Peony Variety Taipinghong

垫江牡丹品种太平红的组织培养研究（英文）

[Objective] This study was to raise the reproductive efficiency of a Dianjiang Peony variety Taipinghong. [Method] The Taipinghong was studied on the best selection and collection time of explant, the induction in the early generation and the subculture multiplication. [Result] The best explant of Taipinghong was bulbil, and the best collecting time was March. The best induction recipe in early generation was modified MS medium （ammonium nitrate rate was reduced by half; calcium ion concentration doubled）＋arrageenan 6 g/L＋succharcose 30 g/L＋NAA 0.2 mg/L＋6-BA 2.0 mg/L＋GA3 0.5 mg/L＋AC 100 mg/L. [Conclusion] In the solution to browning phenomenon, both dark and AC （activated charcoal） treatments could obviously decrease the browning rate of explant.

[目的 ]为提高垫江牡丹品种太平红的增殖率,更好地满足市场供应需求。[方法 ]以太平红品种为试验材料,研究了外植体的选择及其取材时间、初代诱导配方以及继代增殖培养。[结果 ]最佳外植体为鳞芽,取材时间为3月。初代诱导配方为:改良MS培养基(硝酸铵减半,钙离子浓度增加一倍)+NAA 0.2 mg/L+6-BA 2.0 mg/L+GA3 0.5 mg/L+AC 100 mg/L+卡拉胶6 g/L+蔗糖30 g/L。[结论 ]培养的过程中同时进行暗处理和添加AC,对牡丹外植体褐化现象有一定的抑制作用。