四种酚类化合物体外抗氧化活性的比较研究

Study on the Comparison of Antioxidant Activity in Vitro of Four Phenolic Compounds

以L-抗坏血酸棕榈酸酯为对照,采用DPPH自由基清除能力、ABTS自由基清除能力、铁离子还原能力和还原力法,综合评价咖啡酸、芦丁、阿魏酸、槲皮素的抗氧化活性。结果表明:在清除DPPH自由基能力上,咖啡酸和槲皮素的作用均强于阿魏酸、芦丁和L-抗坏血酸棕榈酸酯;在清除ABTS自由基方面,咖啡酸和槲皮素的作用最强,芦丁最弱;在铁离子还原能力和还原力方面,咖啡酸和槲皮素具有很强的还原能力,阿魏酸具有较强的还原能力。酚类化合物的抗氧化活性主要取决于它们含有的酚羟基数目以及化学结构中的位置。

The antioxidant activity of caffeic acid, rutin, ferulic and quercetin was evaluated in vitro using three models of antioxidant including DPPH free radicals scavenging activity, ABTS free radicals scavenging activity, ferric reducing ability and reducing ability. The antioxidant of caffeic acid, rutin, ferulic and quercetin was compared with L-Ascorbic 6-palmitate respectively. The results indicated that caffeic acid and quercetin had strong antioxidant activities on scavenging DPPH free radicals, and the inhibition rate was higher than ferulic, rutin and L-ascorbic 6-palmitate. Caffeic acid and quercetin had the strongest ability on scavenging ABTS free radicals, ferric reducing ability and reducing ability, rutin had the weakest ability on scavenging ABTS free radicals, and ferulic gave the modest reducing ability, respectively. The antioxidant activity of phenolic compounds mainly depended on the number of phenolic hydroxyl groups and the position in the chemical structure.