基于乙酰胆碱酯酶生物传感平台的西维因检测

Acetylcholinesterase Biosensor Platform Based on BP2000 for the Detection of Carbaryl

为给农药西维因检测提供一种新方法,根据西维因抑制乙酰胆碱酯酶活性的原理,以黑珍珠2000(BP2000)为乙酰胆碱酯酶的固定化材料,采用滴凃电极法构建了基于乙酰胆碱酯酶的西维因生物传感平台.结果表明,固定在BP2000上的乙酰胆碱酯酶保持了对氯化乙酰胆碱的催化活性,并且由于BP2000材料的引入,提升了电极有效的电化学活性表面积,而且电极上物质的电化学氧化拥有较低氧化电位(0.630 V)并伴随质子传输.由BP2000搭建成功的乙酰胆碱酯酶生物传感平台对西维因检测的线性响应范围为2.0 ng·m L-1~12.5 ng·m L-1,检测限为3.15 ng·m L-1.本研究对酶生物传感平台和酶生物燃料电池体系中酶电极的构建提供了一种简单方法及高效载体.

With the purpose of providing a new method for carbaryl（a pesticide） detection, on the basis of the principle that acetylcholinesterase（ACh E） activity can be restrained by carbaryl, an ACh E biosensor platform based on BP2000（as a fixation）was constructed by dropping method. As a result, it revealed that ACh E immobilized on BP2000 maintained its catalytic activity for acetylcholine（ATCl）, and due to the introduction of the BP2000 material, the effective electrochemical surface area of the modified electrode was enlarged. In addition, the electrochemical oxidation at the modified electrode occurred at low potential（0.630 V） accompanied by proton transmission. The ACh E biosensor platform based on BP2000 matrix for carbaryl detection was able to reflect a linear response in the range of 2.0 ng·m L-1 - 12.5 ng·m L-1 with the detection limit of 3.15 ng·m L-1. At last, this work will provide a simple method and an efficient matrix in establishing an enzyme electrode of enzymatic biosensor platform and enzymatic fuel cell.