双偏振干涉技术(DPI):生物分子相互作用研究的新工具

Dual polarization interferometry(DPI):a new tool in studying the biomolecular interaction

双偏振干涉(dual polarization interferometry,DPI)技术是近年来发展起来的一种免标记、实时、高灵敏和高分辨率的表面分析技术.它能够精确测量分子相互作用界面层的密度、厚度和质量的绝对值,可实时获取分子相互作用过程的动力学和结构信息.本文简单介绍了DPI的测量原理、仪器组成并对其与相关检测技术的对比进行了简要的概述;着重介绍了近10年来DPI技术在生物分子相互作用研究方面的应用进展,主要包括蛋白质之间以及与其他分子的相互作用,DNA与各种分子之间的相互作用,生物膜与其他分子的相互作用,蛋白质的吸脱附、聚集和结晶过程监测等;并对DPI技术未来的发展进行了展望.随着技术的不断发展,DPI将会在生物分析、纳米材料表征、能源相关表/界面研究等方面得到广泛应用.

Dual polarization interferometry （DPI） is a newly developed label-free, real-time, sensitive and high- resolution surface analysis technique. It allows to accurately determine the layer thickness, mass and density, and to gain the kinetics and structural information during the molecular interaction process. In this review, we first briefly introduce the basic principle of DPI technique, the components of DPI apparatus, and the comparison of DPI with other analysis techniques. Then the applications of DPI in studying the biomolecular interaction are discussed, including the interaction of protein-protein and protein-other molecules, the interaction of DNA with other molecules, the interaction of biomembrane with other molecules, as well as the monitoring of protein adsorption/desorption, aggregation and crystallization processes. The summary and future perspectives are also given in the conclusion.