基于电化学阻抗谱(EIS)的生物细胞检测方法

Measurement of Biological Cells by Electrical Impedance Spectroscopy

本文利用电化学阻抗谱(EIS)对酵母菌细胞的介电性能进行了探究,通过实验,测量了酵母菌细胞悬浮液的电容,并计算出相对介电常数和介电损耗。结果显示,随着酵母菌细胞浓度的增加,相对介电常数和介电损耗也增加。此外,实验结果还显示活细胞的相对介电常数和介电损耗高于死细胞。本研究证明,EIS方法可用来检测细胞悬浮液的状态。

Impedance spectroscopy has been proved to be a useful technique for analyzing heterogeneous systems, especially for biological cell suspensions and tissues because of its capacity of non-invasive measurement. Experiments were conducted to study the dielectric properties of yeast cells by Impedance Spectroscopy. The capacitance of the yeast cell suspension was measured, the relative permittivity and the dielectric loss were calculated. As the concentration of yeast cells increased, the relative permittivity and the dielectric loss were also increased. In addition, the relative permittivity and the dielectric loss of living cells were higher than that of the dead ones. Finally, we concluded that it is possible to sense the status and the concentration of cell suspension by impedance spectroscopy.