一起大学校园内由诺如病毒GⅡ型引起急性胃肠道疫情的病原分子生物学特征分析

Analysis of molecular biological characteristics of an acute gastrointestinal tract incidence caused by norovirus GⅡ in a university campus

目的分析长春市某大学一起胃肠道不适症状事件的诺如病毒分子生物学特征。方法将收集到的104份粪便标本,采用real-time PCR方法检测诺如病毒核酸。阳性标本再进行RT-PCR扩增,扩增产物经过凝胶电泳分析后,进行序列测定,确定基因型,并进行系统发生树分析。结果 104份粪便标本检出44株GⅡ组诺如毒株,22株测序成功,对测序成功的22株毒株进行同源性分析,结果这22株毒株与2016年韩国株Ⅱ.17|KX764738同源关系最近,证实这是一起由GⅡ.17感染引起的聚集性胃肠炎事件。结论此次胃肠炎事件是由诺如病毒引起,且为GⅡ.17毒株感染引起。

Objective To analyze the molecular biological characteristics of norovirus in a university in Changchun. Methods 104 feces specimens were collected,and nucleic acid of norovirus was detected by real-time PCR. The positive samples were amplified by RT-PCR. The amplified products were analyzed by gel electrophoresis,and the genotypes were determined.Phylogenetic tree analysis was performed. Results Forty-four strains of GⅡ norovirus were detected in 104 fecal specimens,and 22 strains were successfully sequenced for homology analysis. The 22 strains had the closest homology with the 2016 Korean strain Ⅱ. 17 ｜ KX764738,confirming that this was a gastroenteritis caused by GⅡ. 17 infection. Conclusion The gastroenteritis was caused by norovirus,especially G Ⅱ. 17 strain.