痛泻要方治疗肠易激综合征肝郁脾虚证VIP作用机制探讨

To discuss the mechanism on serum and colon VIP in rats with ibs liver depression and spleen deficiency treated with Tongxieyaofang

目的:观察痛泻要方对肠易激综合征(IBS)肝郁脾虚证大鼠血清及结肠血管活性肠肽(VIP)水平的影响,探讨痛泻要方治疗肠易激综合征肝郁脾虚证VIP作用机制。方法:运用"束缚应激+番泻叶灌胃法"构造IBS肝郁脾虚证动物模型,运用痛泻要方和匹维溴铵进行治疗,检测各组大鼠血清及结肠VIP的含量,分析痛泻要方治疗IBS肝郁脾虚证VIP作用机制。结果:与正常组相比,病理组血清和结肠VIP含量较正常组明显升高(P<0.01),与病理组相比,低、中、高剂量组及西药组血清和结肠VIP含量显著降低(P<0.05,P<0.01)。结论:痛泻要方治疗肠易激综合征肝郁脾虚证与降低其血清、结肠VIP的含量有关,极有可能是胃肠道动力增强之后而产生的一种负反馈机制造成的。

Objective：To observe the effect on serum and colon VIP in rats with IBS liver depression and spleen deficiency treated with Tongxieyaofang to discuss the its mechanism. Method： 120 SD male rats were divided into normal group,pathology group,Tonxieyaofang Low,medium,high dose group and western medicine group with 20 rats in each group. Except normal group, the other group was made model of IBS liver depression and spleen deficiency by bondage stress and senna lavage. The Tonxieyaofang Low,medium,high dose group and western medicine group were treated with Low, medium, high dose Tonxieyaofang and pinaverium bromide. The VIP of serum and colon VIP in rats was determined and analyzed. Results：Compared with normal group,VIP content in serum and colon of pathological group increased significantly （P〈0.01）;compared with the pathological group, low, serum and colon VIP content of the medium and high dose group and western medicine decreased significantly （P〈0.05,P〈0.01）. Conclusion：The effect of Tongxieyaofang on IBS with liver depression and spleen deficiency is related to the reduction of the content of serum and colon VIP. It may be caused by a negative feedback mechanism after gastrointestinal function enhancement.