不同湿地土壤中真菌群落分析以及纤维素降解菌的筛选

Analysis of Fungal Community Structure and Screening of Cellulose Degrading Fungi in Different Wetland Soils

目的:了解湿地不同植被覆盖土壤真菌群落特征的变化并从中获得高效的纤维素降解菌。方法:采集三个不同植被样地中的土样,利用高通量测序方法测定并通过生物信息学分析土壤真菌群落结构,以纤维素为唯一碳源筛选纤维素降解菌并通过ITS测序进行菌种鉴定。结果:在土壤中共发现6个真菌门和15个优势真菌属,筛选得到的6株产酶能力较强的纤维素降解真菌,分别为木霉(Trichoderma sp.)、青霉(Penicillium sp.)、棘孢曲霉(Aspergillus aculeatus)、尖孢镰刀霉(Fusarium sp.)、黑曲霉(Aspergillusniger)和米曲霉(Aspergilluoryzae)。结论:不同采样点的土壤菌群差异较大,与植被类型、土壤pH、湿度等条件有关,所得降解菌的纤维素降解能力也有很大差异。

Objective： To understand the changes of fungal community structure in different vegetation coverage of wetlands, and obtain efficient cellulose degradation fungi from them. Methods： Soil samples from three different vegetation plots were collected and the fungal community structure was determined by high throughput sequencing and bioinformatics technology. Results： Six fungal divisions and 15 genera were identified in soil. Six strains of cellulolytic fungi were screened from the soil samples, which were Trichoderma sp., Penicillium sp., Aspergillus aculeatus, Fusarium sp., Aspergillusniger and Aspergilluoryzae. Conclusion： Thesoil fungi communitiesamong different sampling sites are varied, which are related to vegetation types, soil p H and humidity. The cellulose degradation ability of degrading fungi is also very different.