CIK细胞的体外扩增能力及杀瘤效应

In Vitro Amplification and Culture of CIK Cells

采用正常志愿者外周血分离单个核细胞,以IL-2、IFN、CD3m Ab诱导CIK细胞,使用流式细胞仪检测细胞表型,MTT法检测研究CIK细胞体外增殖规律、细胞表型变化以及杀瘤活性,结果表明:经过细胞因子诱导产生的CIK细胞成集落快速增殖,16天左右达到增殖高峰,之后增殖速度明显下降,细胞数量开始进入平台期。CD3~?+CD56~?双阳性细胞比例与未经诱导的PBMC细胞相比大幅上升。培养至16天的CIK细胞杀瘤活性强,其杀瘤活性随着效靶比的增加而增强。CIK细胞体外增殖容易,对肿瘤细胞毒性强和毒副作用低,为恶性肿瘤的治疗提供了新的手段。

Objective： To study the proliferationandcultureof CIK cells in vitro. Methods： Mononuclear cells were isolated from peripheral blood of normal volunteers, and CIK cells were induced by IL-2, IFN and CD3 m Ab. Cell phenotype was detected by flow cytometry. Results： CIK cells induced by cytokine induced colonies were rapidly proliferating, and the proportion of CD3^＋ ＋CD56^＋ double positive cells increased significantly compared with that of uninduced PBMC cells. Conclusion： CIK cells are easy to proliferate in vitro, and have strong toxic and side effects on tumor cells, which provide a new means for the treatment of malignant tumors.