摘要
采用正常志愿者外周血分离单个核细胞,以IL-2、IFN、CD3m Ab诱导CIK细胞,使用流式细胞仪检测细胞表型,MTT法检测研究CIK细胞体外增殖规律、细胞表型变化以及杀瘤活性,结果表明:经过细胞因子诱导产生的CIK细胞成集落快速增殖,16天左右达到增殖高峰,之后增殖速度明显下降,细胞数量开始进入平台期。CD3~?+CD56~?双阳性细胞比例与未经诱导的PBMC细胞相比大幅上升。培养至16天的CIK细胞杀瘤活性强,其杀瘤活性随着效靶比的增加而增强。CIK细胞体外增殖容易,对肿瘤细胞毒性强和毒副作用低,为恶性肿瘤的治疗提供了新的手段。
Objective: To study the proliferationandcultureof CIK cells in vitro. Methods: Mononuclear cells were isolated from peripheral blood of normal volunteers, and CIK cells were induced by IL-2, IFN and CD3 m Ab. Cell phenotype was detected by flow cytometry. Results: CIK cells induced by cytokine induced colonies were rapidly proliferating, and the proportion of CD3^+ +CD56^+ double positive cells increased significantly compared with that of uninduced PBMC cells. Conclusion: CIK cells are easy to proliferate in vitro, and have strong toxic and side effects on tumor cells, which provide a new means for the treatment of malignant tumors.
作者
王欣言
Wang Xin-yan(VcanbioBiological Cell Storage Service(Tianjin)Co.,Ltd.,Tianjin30038)
出处
《生物化工》
2018年第4期67-68,72,共3页
Biological Chemical Engineering